Oriented Nanofibrous Polymer Scaffolds Containing Protein-Loaded Porous Silicon Generated by Spray Nebulization

Jonathan M. Zuidema; Tushar Kumeria; Dokyoung Kim; Jinyoung Kang; Joanna Wang; Geoffrey Hollett; Xuan Zhang; David S. Roberts; Nicole Chan; Cari Dowling; Elena Blanco-Suarez; Nicola J. Allen; Mark H. Tuszynski; Michael J. Sailor

doi:10.1002/adma.201706785

Oriented Nanofibrous Polymer Scaffolds Containing Protein-Loaded Porous Silicon Generated by Spray Nebulization

Jonathan M. Zuidema, Tushar Kumeria, Dokyoung Kim, Jinyoung Kang, Joanna Wang, Geoffrey Hollett, Xuan Zhang, David S. Roberts, Nicole Chan, Cari Dowling, Elena Blanco-Suarez, Nicola J. Allen, Mark H. Tuszynski, Michael J. Sailor

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Oriented composite nanofibers consisting of porous silicon nanoparticles (pSiNPs) embedded in a polycaprolactone or poly(lactide-co-glycolide) matrix are prepared by spray nebulization from chloroform solutions using an airbrush. The nanofibers can be oriented by an appropriate positioning of the airbrush nozzle, and they can direct growth of neurites from rat dorsal root ganglion neurons. When loaded with the model protein lysozyme, the pSiNPs allow the generation of nanofiber scaffolds that carry and deliver the protein under physiologic conditions (phosphate-buffered saline (PBS), at 37 °C) for up to 60 d, retaining 75% of the enzymatic activity over this time period. The mass loading of protein in the pSiNPs is 36%, and in the resulting polymer/pSiNP scaffolds it is 3.6%. The use of pSiNPs that display intrinsic photoluminescence (from the quantum-confined Si nanostructure) allows the polymer/pSiNP composites to be definitively identified and tracked by time-gated photoluminescence imaging. The remarkable ability of the pSiNPs to protect the protein payload from denaturation, both during processing and for the duration of the long-term aqueous release study, establishes a model for the generation of biodegradable nanofiber scaffolds that can load and deliver sensitive biologics.

Original language	English
Article number	1706785
Journal	Advanced Materials
Volume	30
Issue number	12
DOIs	https://doi.org/10.1002/adma.201706785
State	Published - Mar 22 2018

Bibliographical note

Publisher Copyright:
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Funding

J.M.Z. and T.K. contributed equally to this work. This work was supported in part by the National Science Foundation, under Grant no. CBET-1603177 (MJS) and by the National Institutes of Health, under Grant no. NINDS-RO1-NS089791 (NJA). J.K. acknowledges financial support from the UCSD Frontiers of Innovation Scholars Program (FISP) fellowship.

Funders	Funder number
U.S. Department of Energy Chinese Academy of Sciences Guangzhou Municipal Science and Technology Project Oak Ridge National Laboratory Extreme Science and Engineering Discovery Environment National Science Foundation National Energy Research Scientific Computing Center National Natural Science Foundation of China	CBET-1603177
U.S. Department of Energy Chinese Academy of Sciences Guangzhou Municipal Science and Technology Project Oak Ridge National Laboratory Extreme Science and Engineering Discovery Environment National Science Foundation National Energy Research Scientific Computing Center National Natural Science Foundation of China
National Institutes of Health (NIH)
Institute of Neurological Disorders and Stroke National Advisory Neurological Disorders and Stroke Council	R01NS089791
Institute of Neurological Disorders and Stroke National Advisory Neurological Disorders and Stroke Council
University of California San Diego Health

Keywords

airbrush
cell guidance
controlled release drug delivery
polycaprolactone
protein therapeutics
time-gated photoluminescence imaging
tissue engineering

ASJC Scopus subject areas

General Materials Science
Mechanics of Materials
Mechanical Engineering

Access to Document

10.1002/adma.201706785

Cite this

Zuidema, J. M., Kumeria, T., Kim, D., Kang, J., Wang, J., Hollett, G., Zhang, X., Roberts, D. S., Chan, N., Dowling, C., Blanco-Suarez, E., Allen, N. J., Tuszynski, M. H., & Sailor, M. J. (2018). Oriented Nanofibrous Polymer Scaffolds Containing Protein-Loaded Porous Silicon Generated by Spray Nebulization. Advanced Materials, 30(12), Article 1706785. https://doi.org/10.1002/adma.201706785

@article{feef41cc41f0480999ee84ee0fc09f0c,

title = "Oriented Nanofibrous Polymer Scaffolds Containing Protein-Loaded Porous Silicon Generated by Spray Nebulization",

abstract = "Oriented composite nanofibers consisting of porous silicon nanoparticles (pSiNPs) embedded in a polycaprolactone or poly(lactide-co-glycolide) matrix are prepared by spray nebulization from chloroform solutions using an airbrush. The nanofibers can be oriented by an appropriate positioning of the airbrush nozzle, and they can direct growth of neurites from rat dorsal root ganglion neurons. When loaded with the model protein lysozyme, the pSiNPs allow the generation of nanofiber scaffolds that carry and deliver the protein under physiologic conditions (phosphate-buffered saline (PBS), at 37 °C) for up to 60 d, retaining 75\% of the enzymatic activity over this time period. The mass loading of protein in the pSiNPs is 36\%, and in the resulting polymer/pSiNP scaffolds it is 3.6\%. The use of pSiNPs that display intrinsic photoluminescence (from the quantum-confined Si nanostructure) allows the polymer/pSiNP composites to be definitively identified and tracked by time-gated photoluminescence imaging. The remarkable ability of the pSiNPs to protect the protein payload from denaturation, both during processing and for the duration of the long-term aqueous release study, establishes a model for the generation of biodegradable nanofiber scaffolds that can load and deliver sensitive biologics.",

keywords = "airbrush, cell guidance, controlled release drug delivery, polycaprolactone, protein therapeutics, time-gated photoluminescence imaging, tissue engineering",

author = "Zuidema, \{Jonathan M.\} and Tushar Kumeria and Dokyoung Kim and Jinyoung Kang and Joanna Wang and Geoffrey Hollett and Xuan Zhang and Roberts, \{David S.\} and Nicole Chan and Cari Dowling and Elena Blanco-Suarez and Allen, \{Nicola J.\} and Tuszynski, \{Mark H.\} and Sailor, \{Michael J.\}",

note = "Publisher Copyright: {\textcopyright} 2018 WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim",

year = "2018",

month = mar,

day = "22",

doi = "10.1002/adma.201706785",

language = "English",

volume = "30",

number = "12",

}

TY - JOUR

T1 - Oriented Nanofibrous Polymer Scaffolds Containing Protein-Loaded Porous Silicon Generated by Spray Nebulization

AU - Zuidema, Jonathan M.

AU - Kumeria, Tushar

AU - Kim, Dokyoung

AU - Kang, Jinyoung

AU - Wang, Joanna

AU - Hollett, Geoffrey

AU - Zhang, Xuan

AU - Roberts, David S.

AU - Chan, Nicole

AU - Dowling, Cari

AU - Blanco-Suarez, Elena

AU - Allen, Nicola J.

AU - Tuszynski, Mark H.

AU - Sailor, Michael J.

PY - 2018/3/22

Y1 - 2018/3/22

N2 - Oriented composite nanofibers consisting of porous silicon nanoparticles (pSiNPs) embedded in a polycaprolactone or poly(lactide-co-glycolide) matrix are prepared by spray nebulization from chloroform solutions using an airbrush. The nanofibers can be oriented by an appropriate positioning of the airbrush nozzle, and they can direct growth of neurites from rat dorsal root ganglion neurons. When loaded with the model protein lysozyme, the pSiNPs allow the generation of nanofiber scaffolds that carry and deliver the protein under physiologic conditions (phosphate-buffered saline (PBS), at 37 °C) for up to 60 d, retaining 75% of the enzymatic activity over this time period. The mass loading of protein in the pSiNPs is 36%, and in the resulting polymer/pSiNP scaffolds it is 3.6%. The use of pSiNPs that display intrinsic photoluminescence (from the quantum-confined Si nanostructure) allows the polymer/pSiNP composites to be definitively identified and tracked by time-gated photoluminescence imaging. The remarkable ability of the pSiNPs to protect the protein payload from denaturation, both during processing and for the duration of the long-term aqueous release study, establishes a model for the generation of biodegradable nanofiber scaffolds that can load and deliver sensitive biologics.

AB - Oriented composite nanofibers consisting of porous silicon nanoparticles (pSiNPs) embedded in a polycaprolactone or poly(lactide-co-glycolide) matrix are prepared by spray nebulization from chloroform solutions using an airbrush. The nanofibers can be oriented by an appropriate positioning of the airbrush nozzle, and they can direct growth of neurites from rat dorsal root ganglion neurons. When loaded with the model protein lysozyme, the pSiNPs allow the generation of nanofiber scaffolds that carry and deliver the protein under physiologic conditions (phosphate-buffered saline (PBS), at 37 °C) for up to 60 d, retaining 75% of the enzymatic activity over this time period. The mass loading of protein in the pSiNPs is 36%, and in the resulting polymer/pSiNP scaffolds it is 3.6%. The use of pSiNPs that display intrinsic photoluminescence (from the quantum-confined Si nanostructure) allows the polymer/pSiNP composites to be definitively identified and tracked by time-gated photoluminescence imaging. The remarkable ability of the pSiNPs to protect the protein payload from denaturation, both during processing and for the duration of the long-term aqueous release study, establishes a model for the generation of biodegradable nanofiber scaffolds that can load and deliver sensitive biologics.

KW - airbrush

KW - cell guidance

KW - controlled release drug delivery

KW - polycaprolactone

KW - protein therapeutics

KW - time-gated photoluminescence imaging

KW - tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=85042034383&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85042034383&partnerID=8YFLogxK

U2 - 10.1002/adma.201706785

DO - 10.1002/adma.201706785

M3 - Article

C2 - 29363828

AN - SCOPUS:85042034383

SN - 0935-9648

VL - 30

JO - Advanced Materials

JF - Advanced Materials

IS - 12

M1 - 1706785

ER -

Oriented Nanofibrous Polymer Scaffolds Containing Protein-Loaded Porous Silicon Generated by Spray Nebulization

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this