Oropouche virus infection and pathogenesis are restricted by MAVs, IRF-3, IRF-7, and type I interferon signaling pathways in nonmyeloid cells

Jose Luiz Proenca-Modena, Renata Sesti-Costa, Amelia K. Pinto, Justin M. Richner, Helen M. Lazear, Tiffany Lucas, Jennifer L. Hyde, Michael S. Diamond

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Oropouche virus (OROV) is a member of the Orthobunyavirus genus in the Bunyaviridae family and a prominent cause of insect- transmitted viral disease in Central and South America. Despite its clinical relevance, little is known about OROV pathogenesis. To define the host defense pathways that control OROV infection and disease, we evaluated OROV pathogenesis and immune responses in primary cells and mice that were deficient in the RIG-I-like receptor signaling pathway (MDA5, RIG-I, or MAVS), downstream regulatory transcription factors (IRF-3 or IRF-7), beta interferon (IFN-β), or the receptor for type I IFN signaling (IFNAR). OROV replicated to higher levels in primary fibroblasts and dendritic cells lacking MAVS signaling, the transcription factors IRF-3 and IRF-7, or IFNAR than in wild-type (WT) cells. In mice, deletion of IFNAR, MAVS, or IRF-3 and IRF-7 resulted in uncontrolled OROV replication, hypercytokinemia, extensive liver damage, and death, whereas WT congenic animals failed to develop disease. Unexpectedly, mice with a selective deletion of IFNAR on myeloid cells (CD11c Cre+ Ifnarf/f or LysM Cre+ Ifnarf/f) did not sustain enhanced disease with OROV or a selective (flox/flox) deletion La Crosse virus, a closely related encephalitic orthobunyavirus. In bone marrow chimera studies, recipient irradiated Ifnar-/- mice reconstituted with WT hematopoietic cells sustained high levels of OROV replication and liver damage, whereas WT mice reconstituted with Ifnar-/- bone marrow were resistant to disease. Collectively, these results establish a dominant protective role for MAVS, IRF-3 and IRF-7, and IFNAR in restricting OROV infection and tissue injury and suggest that IFN signaling in nonmyeloid cells contributes to the host defense against orthobunyaviruses.

Original languageEnglish
Pages (from-to)4720-4737
Number of pages18
JournalJournal of Virology
Issue number9
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© 2015, American Society for Microbiology.

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology


Dive into the research topics of 'Oropouche virus infection and pathogenesis are restricted by MAVs, IRF-3, IRF-7, and type I interferon signaling pathways in nonmyeloid cells'. Together they form a unique fingerprint.

Cite this