OsIPD3, an ortholog of the Medicago truncatula DMI3 interacting protein IPD3, is required for mycorrhizal symbiosis in rice

Caiyan Chen, Jean Michel Ané, Hongyan Zhu

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

• Medicago truncatula IPD3 (MtIPD3) is an interacting protein of DMI3 (does not make infections 3), a Ca2+/calmodulin-dependent protein kinase (CCaMK) essential for both arbuscular mycorrhizal (AM) and rhizobial symbioses. However, the function of MtIPD3 in root symbioses has not been demonstrated in M. truncatula, because of a lack of knockout mutants for functional analysis. In this study, the availability of IPD3 knockout mutants in rice (Oryza sativa) was exploited to test the function of OsIPD3 in AM symbiosis. • Three independent retrotransposon Tos17 insertion lines of OsIPD3 were selected and the phenotypes characterized upon inoculation with the AM fungus Glomus intraradices. • Phenotypic and genetic analyses revealed that the Osipd3 mutants were unable to establish a symbiotic association with G. intraradices. • In conclusion, IPD3 represents a novel gene required for root symbiosis with AM fungi in plants.

Original languageEnglish
Pages (from-to)311-315
Number of pages5
JournalNew Phytologist
Volume180
Issue number2
DOIs
StatePublished - Oct 2008

Keywords

  • DMI3
  • IPD3
  • Medicago truncatula
  • Mycorrhizal symbiosis
  • Nodulation
  • Rice (Oryza sativa)

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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