Ovarian prostaglandin synthase: Immunohistochemical localization in the rat

Thomas E. Curry; Anita Malik; Martin R. Clark

doi:10.1016/S0002-9378(87)80003-0

Ovarian prostaglandin synthase: Immunohistochemical localization in the rat

Thomas E. Curry, Anita Malik, Martin R. Clark

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Ovarian prostaglandin synthase is stimulated by the luteinizing hormone surge resulting in the preovulatory increase in prostaglandins. In the present study, the ovarian cellular localization of prostaglandin synthase was identified by immunohistochemistry. Ovaries were collected from 27-day-old rats at the time of stimulation with pregnant mare serum gonadotropin (8 IU) (zero hours), 48 hours later, or 8 hours after administration of human chorionic gonadotropin (5 IU). At zero hours prostaglandin synthase immunostaining was present in the theca of larger follicles and interstitial regions. The number and intensity of immunostained cells in the theca increased between zero and 48 hours. The granulosa cell layer adjacent to the basement membrane in large an tral follicles exhibited immunostaining. A similar staining pattern was observed 8 hours after human chorionic gonadotropin. These observations indicate that in the rat, the theca, interstitium, and granulosa contain prostaglandin synthase immunoreactivity and may contribute prostaglandins during follicular development and ovulation.

Original language	English
Pages (from-to)	537-543
Number of pages	7
Journal	American Journal of Obstetrics and Gynecology
Volume	157
Issue number	3
DOIs	https://doi.org/10.1016/S0002-9378(87)80003-0
State	Published - 1987

Bibliographical note

Funding Information:
From the Reproductive Sciences and Endocrinology Laboratories, De-partment of Obstetrics and Gynecology, University of Miami. This work was supported in part by National Institutes of Health Grants HD-13073 and HD-22299 and a john R. McCain Stu-dent Fellowship to A. M. by the South Atlantic Association of Ob-stetricians and Gynecologists. john R. McCain Student Fellowship Award Presentation, presented at the Forty-ninth Annual Meeting of The South Atlantic Associ-ation of Obstetricians and Gynecologists, Hot Springs, Virginia, January 25-28, 1987. Reprint requests: Thomas E. Curry, Jr., The Kentucky Center for Reproductive Medicine, Department of Obstetrics and Gynecology, University of Kentucky, Lexington, KY 40536.

Funding Information:
The didactic criticisms of Dr. William LeMaire throughout all stages of this project are gratefully acknowledged. The assistance of Drs. Mehrdad Nadji and Jocelyne Weissman of the Department of Pathology and the technical help of Brad Ress were fundamental during the early stages of immunohistochemical optimization. We appreciate the provision of pregnant mare serum gonadotropin by the National Hormone and Pituitary Program, National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases.

Keywords

Prostaglandins
follicle
ovary
ovulation
prostaglandin synthase

ASJC Scopus subject areas

Obstetrics and Gynecology

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10.1016/S0002-9378(87)80003-0

Cite this

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title = "Ovarian prostaglandin synthase: Immunohistochemical localization in the rat",

abstract = "Ovarian prostaglandin synthase is stimulated by the luteinizing hormone surge resulting in the preovulatory increase in prostaglandins. In the present study, the ovarian cellular localization of prostaglandin synthase was identified by immunohistochemistry. Ovaries were collected from 27-day-old rats at the time of stimulation with pregnant mare serum gonadotropin (8 IU) (zero hours), 48 hours later, or 8 hours after administration of human chorionic gonadotropin (5 IU). At zero hours prostaglandin synthase immunostaining was present in the theca of larger follicles and interstitial regions. The number and intensity of immunostained cells in the theca increased between zero and 48 hours. The granulosa cell layer adjacent to the basement membrane in large an tral follicles exhibited immunostaining. A similar staining pattern was observed 8 hours after human chorionic gonadotropin. These observations indicate that in the rat, the theca, interstitium, and granulosa contain prostaglandin synthase immunoreactivity and may contribute prostaglandins during follicular development and ovulation.",

keywords = "Prostaglandins, follicle, ovary, ovulation, prostaglandin synthase",

author = "Curry, {Thomas E.} and Anita Malik and Clark, {Martin R.}",

note = "Funding Information: From the Reproductive Sciences and Endocrinology Laboratories, De-partment of Obstetrics and Gynecology, University of Miami. This work was supported in part by National Institutes of Health Grants HD-13073 and HD-22299 and a john R. McCain Stu-dent Fellowship to A. M. by the South Atlantic Association of Ob-stetricians and Gynecologists. john R. McCain Student Fellowship Award Presentation, presented at the Forty-ninth Annual Meeting of The South Atlantic Associ-ation of Obstetricians and Gynecologists, Hot Springs, Virginia, January 25-28, 1987. Reprint requests: Thomas E. Curry, Jr., The Kentucky Center for Reproductive Medicine, Department of Obstetrics and Gynecology, University of Kentucky, Lexington, KY 40536. Funding Information: The didactic criticisms of Dr. William LeMaire throughout all stages of this project are gratefully acknowledged. The assistance of Drs. Mehrdad Nadji and Jocelyne Weissman of the Department of Pathology and the technical help of Brad Ress were fundamental during the early stages of immunohistochemical optimization. We appreciate the provision of pregnant mare serum gonadotropin by the National Hormone and Pituitary Program, National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases.",

year = "1987",

doi = "10.1016/S0002-9378(87)80003-0",

language = "English",

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AU - Malik, Anita

AU - Clark, Martin R.

N1 - Funding Information: From the Reproductive Sciences and Endocrinology Laboratories, De-partment of Obstetrics and Gynecology, University of Miami. This work was supported in part by National Institutes of Health Grants HD-13073 and HD-22299 and a john R. McCain Stu-dent Fellowship to A. M. by the South Atlantic Association of Ob-stetricians and Gynecologists. john R. McCain Student Fellowship Award Presentation, presented at the Forty-ninth Annual Meeting of The South Atlantic Associ-ation of Obstetricians and Gynecologists, Hot Springs, Virginia, January 25-28, 1987. Reprint requests: Thomas E. Curry, Jr., The Kentucky Center for Reproductive Medicine, Department of Obstetrics and Gynecology, University of Kentucky, Lexington, KY 40536. Funding Information: The didactic criticisms of Dr. William LeMaire throughout all stages of this project are gratefully acknowledged. The assistance of Drs. Mehrdad Nadji and Jocelyne Weissman of the Department of Pathology and the technical help of Brad Ress were fundamental during the early stages of immunohistochemical optimization. We appreciate the provision of pregnant mare serum gonadotropin by the National Hormone and Pituitary Program, National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases.

PY - 1987

Y1 - 1987

N2 - Ovarian prostaglandin synthase is stimulated by the luteinizing hormone surge resulting in the preovulatory increase in prostaglandins. In the present study, the ovarian cellular localization of prostaglandin synthase was identified by immunohistochemistry. Ovaries were collected from 27-day-old rats at the time of stimulation with pregnant mare serum gonadotropin (8 IU) (zero hours), 48 hours later, or 8 hours after administration of human chorionic gonadotropin (5 IU). At zero hours prostaglandin synthase immunostaining was present in the theca of larger follicles and interstitial regions. The number and intensity of immunostained cells in the theca increased between zero and 48 hours. The granulosa cell layer adjacent to the basement membrane in large an tral follicles exhibited immunostaining. A similar staining pattern was observed 8 hours after human chorionic gonadotropin. These observations indicate that in the rat, the theca, interstitium, and granulosa contain prostaglandin synthase immunoreactivity and may contribute prostaglandins during follicular development and ovulation.

AB - Ovarian prostaglandin synthase is stimulated by the luteinizing hormone surge resulting in the preovulatory increase in prostaglandins. In the present study, the ovarian cellular localization of prostaglandin synthase was identified by immunohistochemistry. Ovaries were collected from 27-day-old rats at the time of stimulation with pregnant mare serum gonadotropin (8 IU) (zero hours), 48 hours later, or 8 hours after administration of human chorionic gonadotropin (5 IU). At zero hours prostaglandin synthase immunostaining was present in the theca of larger follicles and interstitial regions. The number and intensity of immunostained cells in the theca increased between zero and 48 hours. The granulosa cell layer adjacent to the basement membrane in large an tral follicles exhibited immunostaining. A similar staining pattern was observed 8 hours after human chorionic gonadotropin. These observations indicate that in the rat, the theca, interstitium, and granulosa contain prostaglandin synthase immunoreactivity and may contribute prostaglandins during follicular development and ovulation.

KW - Prostaglandins

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JO - American Journal of Obstetrics and Gynecology

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IS - 3

ER -

Ovarian prostaglandin synthase: Immunohistochemical localization in the rat

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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