Over-expression of CP9 and CP83 increases whitefly cell cuticle thickness leading to imidacloprid resistance

Chao He; Jinjin Liang; Jing Yang; Hu Xue; Mingjiao Huang; Buli Fu; Xuegao Wei; Shaonan Liu; Tianhua Du; Yao Ji; Cheng Yin; Peipan Gong; Jin Yu Hu; He Du; Rong Zhang; Wen Xie; Shaoli Wang; Qingjun Wu; Xuguo Zhou; Xin Yang; Youjun Zhang

doi:10.1016/j.ijbiomac.2023.123647

Over-expression of CP9 and CP83 increases whitefly cell cuticle thickness leading to imidacloprid resistance

Chao He, Jinjin Liang, Jing Yang, Hu Xue, Mingjiao Huang, Buli Fu, Xuegao Wei, Shaonan Liu, Tianhua Du, Yao Ji, Cheng Yin, Peipan Gong, Jin Yu Hu, He Du, Rong Zhang, Wen Xie, Shaoli Wang, Qingjun Wu, Xuguo Zhou, Xin YangYoujun Zhang

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Cuticular proteins (CPs) play an important role in protecting insects from adverse environmental conditions, like neonicotinoid insecticides, which are heavily used for numerous pests and caused environmental problems and public health concerns worldwide. However, the relationship between CPs and insecticides resistance in Bemisia tabaci, a serious and developed high insecticide resistance, is lacking. In this study, 125 CPs genes were identified in B. tabaci. Further phylogenetic tree showed the RR-2-type genes formed large gene groups in B. tabaci. Transcriptional expression levels of CPs genes at different developmental stages revealed that some CPs genes may play a specific role in insect development. The TEM results indicated that the cuticle thickness of susceptible strain was thinner than imidacloprid-resistance strain. Furthermore, 16 CPs genes (5 in RR-1 subfamily, 7 in RR-2 subfamily, 3 in CPAP3 subfamily and 1 in CPCFC subfamily) were activated in response to imidacloprid. And RNAi results indicated that CP9 and CP83 involved in imidacloprid resistance. In conclusion, this study was the first time to establish a basic information framework and evolutionary relationship between CPs and imidacloprid resistance in B. tabaci, which provides a basis for proposing integrated pest management strategies.

Original language	English
Article number	123647
Journal	International Journal of Biological Macromolecules
Volume	233
DOIs	https://doi.org/10.1016/j.ijbiomac.2023.123647
State	Published - Apr 1 2023

Bibliographical note

Publisher Copyright:
© 2023

Funding

This research was supported by the National Natural Science Foundation of China ( 32161143020 , 32122073 ), Beijing Natural Science Foundation ( 6212031 ), China Agriculture Research System ( CARS-24-C-02 ), The 2020 Research Program of Sanya Yazhou Bay Science and Technology City ( SKJC -2020-02-012 ). The Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables and the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS).

Funders	Funder number
Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables
CAAS-ASTIP-IVFCAAS
National Natural Science Foundation of China (NSFC)	32122073, 32161143020
National Natural Science Foundation of China (NSFC)
Natural Science Foundation of Beijing Municipality	6212031
Natural Science Foundation of Beijing Municipality
Chinese Academy of Agricultural Sciences
Agriculture Research System of China	SKJC -2020-02-012, CARS-24-C-02
Agriculture Research System of China

Keywords

Bemisia tabaci
Cuticular proteins
Expression patterns
Imidacloprid

ASJC Scopus subject areas

Structural Biology
Biochemistry
Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ijbiomac.2023.123647

Cite this

He, C., Liang, J., Yang, J., Xue, H., Huang, M., Fu, B., Wei, X., Liu, S., Du, T., Ji, Y., Yin, C., Gong, P., Hu, J. Y., Du, H., Zhang, R., Xie, W., Wang, S., Wu, Q., Zhou, X., ... Zhang, Y. (2023). Over-expression of CP9 and CP83 increases whitefly cell cuticle thickness leading to imidacloprid resistance. International Journal of Biological Macromolecules, 233, Article 123647. https://doi.org/10.1016/j.ijbiomac.2023.123647

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title = "Over-expression of CP9 and CP83 increases whitefly cell cuticle thickness leading to imidacloprid resistance",

abstract = "Cuticular proteins (CPs) play an important role in protecting insects from adverse environmental conditions, like neonicotinoid insecticides, which are heavily used for numerous pests and caused environmental problems and public health concerns worldwide. However, the relationship between CPs and insecticides resistance in Bemisia tabaci, a serious and developed high insecticide resistance, is lacking. In this study, 125 CPs genes were identified in B. tabaci. Further phylogenetic tree showed the RR-2-type genes formed large gene groups in B. tabaci. Transcriptional expression levels of CPs genes at different developmental stages revealed that some CPs genes may play a specific role in insect development. The TEM results indicated that the cuticle thickness of susceptible strain was thinner than imidacloprid-resistance strain. Furthermore, 16 CPs genes (5 in RR-1 subfamily, 7 in RR-2 subfamily, 3 in CPAP3 subfamily and 1 in CPCFC subfamily) were activated in response to imidacloprid. And RNAi results indicated that CP9 and CP83 involved in imidacloprid resistance. In conclusion, this study was the first time to establish a basic information framework and evolutionary relationship between CPs and imidacloprid resistance in B. tabaci, which provides a basis for proposing integrated pest management strategies.",

keywords = "Bemisia tabaci, Cuticular proteins, Expression patterns, Imidacloprid",

author = "Chao He and Jinjin Liang and Jing Yang and Hu Xue and Mingjiao Huang and Buli Fu and Xuegao Wei and Shaonan Liu and Tianhua Du and Yao Ji and Cheng Yin and Peipan Gong and Hu, {Jin Yu} and He Du and Rong Zhang and Wen Xie and Shaoli Wang and Qingjun Wu and Xuguo Zhou and Xin Yang and Youjun Zhang",

note = "Publisher Copyright: {\textcopyright} 2023",

year = "2023",

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day = "1",

doi = "10.1016/j.ijbiomac.2023.123647",

language = "English",

volume = "233",

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He, C, Liang, J, Yang, J, Xue, H, Huang, M, Fu, B, Wei, X, Liu, S, Du, T, Ji, Y, Yin, C, Gong, P, Hu, JY, Du, H, Zhang, R, Xie, W, Wang, S, Wu, Q, Zhou, X, Yang, X & Zhang, Y 2023, 'Over-expression of CP9 and CP83 increases whitefly cell cuticle thickness leading to imidacloprid resistance', International Journal of Biological Macromolecules, vol. 233, 123647. https://doi.org/10.1016/j.ijbiomac.2023.123647

TY - JOUR

T1 - Over-expression of CP9 and CP83 increases whitefly cell cuticle thickness leading to imidacloprid resistance

AU - He, Chao

AU - Liang, Jinjin

AU - Yang, Jing

AU - Xue, Hu

AU - Huang, Mingjiao

AU - Fu, Buli

AU - Wei, Xuegao

AU - Liu, Shaonan

AU - Du, Tianhua

AU - Ji, Yao

AU - Yin, Cheng

AU - Gong, Peipan

AU - Hu, Jin Yu

AU - Du, He

AU - Zhang, Rong

AU - Xie, Wen

AU - Wang, Shaoli

AU - Wu, Qingjun

AU - Zhou, Xuguo

AU - Yang, Xin

AU - Zhang, Youjun

PY - 2023/4/1

Y1 - 2023/4/1

N2 - Cuticular proteins (CPs) play an important role in protecting insects from adverse environmental conditions, like neonicotinoid insecticides, which are heavily used for numerous pests and caused environmental problems and public health concerns worldwide. However, the relationship between CPs and insecticides resistance in Bemisia tabaci, a serious and developed high insecticide resistance, is lacking. In this study, 125 CPs genes were identified in B. tabaci. Further phylogenetic tree showed the RR-2-type genes formed large gene groups in B. tabaci. Transcriptional expression levels of CPs genes at different developmental stages revealed that some CPs genes may play a specific role in insect development. The TEM results indicated that the cuticle thickness of susceptible strain was thinner than imidacloprid-resistance strain. Furthermore, 16 CPs genes (5 in RR-1 subfamily, 7 in RR-2 subfamily, 3 in CPAP3 subfamily and 1 in CPCFC subfamily) were activated in response to imidacloprid. And RNAi results indicated that CP9 and CP83 involved in imidacloprid resistance. In conclusion, this study was the first time to establish a basic information framework and evolutionary relationship between CPs and imidacloprid resistance in B. tabaci, which provides a basis for proposing integrated pest management strategies.

AB - Cuticular proteins (CPs) play an important role in protecting insects from adverse environmental conditions, like neonicotinoid insecticides, which are heavily used for numerous pests and caused environmental problems and public health concerns worldwide. However, the relationship between CPs and insecticides resistance in Bemisia tabaci, a serious and developed high insecticide resistance, is lacking. In this study, 125 CPs genes were identified in B. tabaci. Further phylogenetic tree showed the RR-2-type genes formed large gene groups in B. tabaci. Transcriptional expression levels of CPs genes at different developmental stages revealed that some CPs genes may play a specific role in insect development. The TEM results indicated that the cuticle thickness of susceptible strain was thinner than imidacloprid-resistance strain. Furthermore, 16 CPs genes (5 in RR-1 subfamily, 7 in RR-2 subfamily, 3 in CPAP3 subfamily and 1 in CPCFC subfamily) were activated in response to imidacloprid. And RNAi results indicated that CP9 and CP83 involved in imidacloprid resistance. In conclusion, this study was the first time to establish a basic information framework and evolutionary relationship between CPs and imidacloprid resistance in B. tabaci, which provides a basis for proposing integrated pest management strategies.

KW - Bemisia tabaci

KW - Cuticular proteins

KW - Expression patterns

KW - Imidacloprid

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U2 - 10.1016/j.ijbiomac.2023.123647

DO - 10.1016/j.ijbiomac.2023.123647

M3 - Article

C2 - 36780959

AN - SCOPUS:85147932538

SN - 0141-8130

VL - 233

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

M1 - 123647

ER -

Over-expression of CP9 and CP83 increases whitefly cell cuticle thickness leading to imidacloprid resistance

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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