TY - JOUR
T1 - Overexpression of the neurotrophic cytokine S100β in human temporal lobe epilepsy
AU - Griffin, W. S.T.
AU - Yeralan, O.
AU - Sheng, J. G.
AU - Boop, F. A.
AU - ak, R. E.
AU - Rovnaghi, C. R.
AU - Burnett, B. A.
AU - Feoktistova, A.
AU - Van Eldik, L. J.
PY - 1995/7
Y1 - 1995/7
N2 - Neuritic sprouting and disturbances of calcium homeostasis are well described in epilepsy. S100β is an astrocyte-derived cytokine that promotes neurite growth and induces increases in levels of intracellular calcium in neurons. In sections of neocortex of surgically resected temporal lobe tissue from patients with intractable epilepsy, we found that the number of S100β- immunoreactive astrocytes was approximately threefold higher than that found in control patients (p < 0.001). These astrocytes were activated, i.e., enlarged, and had prominent processes. Temporal lobe tissue levels of S100β were shown by ELISA to be fivefold higher in 21 epileptics than in 12 controls (p < 0.001). The expression of the astrocyte intermediate filament protein, glial fibrillary acidic protein, was not significantly elevated in epileptics, suggesting a selective up-regulation of S100β expression. Our findings, together with established functions of S100β, suggest that this neurotrophic cytokine may be involved in the pathophysiology of epilepsy.
AB - Neuritic sprouting and disturbances of calcium homeostasis are well described in epilepsy. S100β is an astrocyte-derived cytokine that promotes neurite growth and induces increases in levels of intracellular calcium in neurons. In sections of neocortex of surgically resected temporal lobe tissue from patients with intractable epilepsy, we found that the number of S100β- immunoreactive astrocytes was approximately threefold higher than that found in control patients (p < 0.001). These astrocytes were activated, i.e., enlarged, and had prominent processes. Temporal lobe tissue levels of S100β were shown by ELISA to be fivefold higher in 21 epileptics than in 12 controls (p < 0.001). The expression of the astrocyte intermediate filament protein, glial fibrillary acidic protein, was not significantly elevated in epileptics, suggesting a selective up-regulation of S100β expression. Our findings, together with established functions of S100β, suggest that this neurotrophic cytokine may be involved in the pathophysiology of epilepsy.
KW - Astrocyte
KW - ELISA
KW - Epilepsy
KW - Glial fibrillary acidic protein
KW - Immunohistochemistry
KW - S100
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M3 - Article
C2 - 7790864
AN - SCOPUS:0029061066
SN - 0022-3042
VL - 65
SP - 228
EP - 233
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 1
ER -