Carcinoid tumors are rare neuroendocrine tumors with a predilection for the gastrointestinal tract. Protein kinase D (PKD), a novel serine/threonine protein kinase, has been implicated in the regulation of transport processes in certain cell types. We have reported an important role for PKD in stimulated peptide secretion from a human (BON) carcinoid cell line; however, the role of PKD isoforms, including PKD2, in the proliferation and invasion of carcinoid tumors remains unclear. In the present study, we found that overexpression of PKD2 by stable transfection of BON cells with PKD2-wild type (PKD2WT) significantly increased proliferation and invasion compared to cells transfected with PKD2-kinase dead (PKD2KD) or pcDNA3 (control). Similarly, inhibition of PKD2 activity with small interfering RNA (siRNA) significantly decreased proliferation and invasion compared to cells transfected with non-targeting control (NTC) siRNA. These data support an important role for PKD2 in carcinoid tumor progression. Targeted inhibition of the PKD family may prove to be a novel treatment option for patients with carcinoid tumors.
|Number of pages||5|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Sep 29 2006|
Bibliographical noteFunding Information:
The authors thank Dr. Kathleen O’Connor for thoughtful comments, Zobeida Cruz-Monserrate for technical assistance, Emily Bayne and Andrea Ramirez for maintenance of the BON cell line, Karen Martin for manuscript preparation, and Tatsuo Uchida for statistical analysis. This work was supported by Grants R01DK48498, R37AG10885, P01DK35608, and T32DK07639 from the National Institutes of Health and a Jeane B. Kempner Scholar award (to L.N.J.).
- BON cell line
- Protein kinase D2 (PKD2)
- Small interfering RNA (siRNA)
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology