Overexpression of XIAP inhibits apoptotic cell death in an oligodendroglial cell line

Ying Jin, Melanie L. McEwen, M. Said Ghandour, Joe E. Springer

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

1. This study describes the use of an oligodendroglial cell line (158N) to study the protective effects of X-chromosome-linked inhibitor of apoptosis (XIAP) overexpression. 2. 158N cells were transiently transfected with either pCMV-Myc-XIAP or control pCMV-Myc vector. At 48 h post-transfection, immunoblotting and immunocytochemical staining snowed robust myc-XIAP overexpression in pCMV-Myc-XIAP transfected cells relative to pCMV-Myc-transfected cells and normal 158N cells. 158N cells were treated with either 100 nm staurosporine (STS) or 300 μM dopamine (DA) and cell survival/function determined using two cell viability assays. 3. Both STS and DA treatments resulted in increased apoptotic death of pCMV-Myc transfected cells. In contrast, there was significant decrease in apoptotic cell death in cells transfected with pCMV-Myc-XIAP. Finally. XIAP overexpression was found to significantly reduce caspase-3 enzyme activity levels in response to apoptotic stimuli. 4. These results provide evidence that XIAP overexpression promotes cell survival in a non-neuronal cell type derived from the central nervous system. In addition, these data suggest that the 158N oligodendroglial cell line is a suitable tool for transient transfection studies, which is a problem frequently encountered when attempting to introduce genes of interest in cultures of primary oligodendroglia.

Original languageEnglish
Pages (from-to)853-863
Number of pages11
JournalCellular and Molecular Neurobiology
Volume24
Issue number6
DOIs
StatePublished - Dec 2004

Keywords

  • Apoptosis
  • Demyelination
  • IAPs, spinal cord injury, multiple sclerosis

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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