Ovulations in rat ovaries perfused in vitro with follicle-stimulating hormone

M. Shaykh, W. J. LeMaire, H. Papkoff, T. E. Curry, J. H. Sogn, R. D. Koos

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13 Scopus citations


Using the model of the isolated perfused rat ovary, we have found that highly purified ovine follicle-stimulating hormone (FSH) preparations cause ovulation and that this effect is not due to luteinizing hormone induced contamination. Ovine FSH-13 at a concentration of 1.5 mU/ml (induced ovulations in all perfused ovaries (8.8±2.3 ovulations/ovary, as did a more purified preparation, ovine FSH-211B, at concentrations of 0.5mU/ml (15.0±6.4 ovulations/ovary) and 5mU/ml (11.3±2.6 ovulations/ovary). This ovulation-inducing effect of FSH is accompanied by a marked stimulation of estradiol levels in the perfusion medium without stimulation of progesterone levels. Furthermore, a purified rat FSH preparation (15 mU/ml also induced ovulation in all ovaries (13.8±2.2 ovulations/ovary) as well as a stimulation of both estradiol and progesterone in the medium. These data clearly confirm the direct ovulatory effect of FSH on the ovary.

Original languageEnglish
Pages (from-to)629-636
Number of pages8
JournalBiology of Reproduction
Issue number3
StatePublished - 1985

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology


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