Oxidative DNA damage in mild cognitive impairment and late-stage Alzheimer's disease

Mark A. Lovell, William R. Markesbery

Research output: Contribution to journalArticlepeer-review

401 Scopus citations

Abstract

Increasing evidence supports a role for oxidative DNA damage in aging and several neurodegenerative diseases including Alzheimer's disease (AD). Attack of DNA by reactive oxygen species (ROS), particularly hydroxyl radicals, can lead to strand breaks, DNA-DNA and DNA-protein cross-linking, and formation of at least 20 modified bases adducts. In addition, α,β-unsaturated aldehydic by-products of lipid peroxidation including 4-hydroxynonenal and acrolein can interact with DNA bases leading to the formation of bulky exocyclic adducts. Modification of DNA bases by direct interaction with ROS or aldehydes can lead to mutations and altered protein synthesis. Several studies of DNA base adducts in late-stage AD (LAD) brain show elevations of 8-hydroxyguanine (8-OHG), 8-hydroxyadenine (8-OHA), 5-hydroxycytosine (5-OHC), and 5-hydroxyuracil, a chemical degradation product of cytosine, in both nuclear and mitochondrial DNA (mtDNA) isolated from vulnerable regions of LAD brain compared to age-matched normal control subjects. Previous studies also show elevations of acrolein/guanine adducts in the hippocampus of LAD subjects compared to age-matched controls. In addition, studies of base excision repair show a decline in repair of 8-OHG in vulnerable regions of LAD brain. Our recent studies show elevated 8-OHG, 8-OHA, and 5,6-diamino-5-formamidopyrimidine in both nuclear and mtDNA isolated from vulnerable brain regions in amnestic mild cognitive impairment, the earliest clinical manifestation of AD, suggesting that oxidative DNA damage is an early event in AD and is not merely a secondary phenomenon.

Original languageEnglish
Pages (from-to)7497-7504
Number of pages8
JournalNucleic Acids Research
Volume35
Issue number22
DOIs
StatePublished - Dec 2007

Bibliographical note

Funding Information:
Work in the authors’ laboratories was funded by grants from the National Institute on Aging (P30-AG0-28383, 5P01-AG0-5119) and a grant from the Abercrombie Foundation. The authors thank Paula Thomason for editorial assistance and Geri Gerke for assistance with figure preparation. Funding to pay the Open Access publication charges for this article was provided by the Abercrombie Foundation.

ASJC Scopus subject areas

  • Genetics

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