Free oxygen radicals are involved in the pathogenesis of necrotizing enterocolitis (NEC) in premature infants. The stress-activated p38 mitogen-activated protein kinase (MAPK) has been implicated in gut injury. Here, we found that phosphorylated p38 was detected primarily in the villus tips of normal intestine, whereas it was expressed in the entire mucosa in NEC. H2O2 treatment resulted in a rapid phosphorylation of p38 MAPK and subsequent apoptosis of rat intestinal epithelial (RIE)-1 cells; this induction was attenuated by treatment with SB203580, a selective p38 MAPK inhibitor, or transfection with p38α siRNA. Moreover, SB203580 also blocked H2O2-induced PKC activation. In contrast, the PKC inhibitor (GF109203x) did not affect p38 activation, indicating that p38 MAPK activation occurs upstream of PKC activation in H2O2-induced apoptosis. H2O2 treatment also decreased mitochondrial membrane potential; pretreatment with SB203580 attenuated this response. Our study demonstrates that the p38 MAPK/PKC pathway plays an important role as a pro-apoptotic cellular signaling during oxidative stress-induced intestinal epithelial cell injury.
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Dec 1 2006|
Bibliographical noteFunding Information:
The authors thank Karen Martin for manuscript preparation and Tatsuo Uchida for assistance with statistical analysis. This work was supported by Grants RO1 DK61470, RO1 DK48498, and PO1 DK35608 from the National Institutes of Health and 8580 from the Shriners Burns Hospital.
- Intestinal epithelial cell injury
- Oxidative stress
- p38 MAPK
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology