Oxidized lipid-mediated alterations in proteoglycan metabolism in cultured pulmonary endothelial cells

Santhini Ramasamy, David W. Lipke, Gibert A. Boissonneault, Hongtao Guo, Bernhard Hennig

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Compared to cholesterol or linoleic acid (18:2), oxidized lipids such as cholestan-3β,5α,6β-triol (triol) and hydroperoxy linoleic acid (HPODE) markedly impair endothelial barrier function in culture [Hennig and Boissonneault, 1987; Hennig et al. 1986]. Because proteoglycans contribute to vascular permeability properties, the effects of cholesterol and 18:2 and their oxidation products, triol and HPODE, on endothelial proteoglycan metabolism were determined. While cholesterol was without effect, a concentration-dependent decrease in cellular proteoglycans (measured by 35S incorporation) was observed after exposure to triol. Compared to control cultures, cholesterol reduced mRNA levels for the proteoglycans, perlecan and biglycan. Triol had a similar effect on biglycan but not on perlecan mRNA levels. Compared to 18:2, 1, 3 and 5 μM HPODE depressed cellular proteoglycans. Perlecan mRNA levels were reduced more by HPODE when compared to 18:2. Biglycan mRNA levels were reduced by 3 μM, but not by 5 μM HPODE. These data demonstrate that oxidized lipids such as triol and HPODE can decrease cellular proteoglycan metabolism in endothelial monolayers and alter mRNA levels of major specific proteoglycans in a concentration-dependent manner. This may have implications in lipid-mediated disruption of endothelial barrier function and atherosclerosis.

Original languageEnglish
Pages (from-to)199-208
Number of pages10
Issue number1-2
StatePublished - Feb 1996

Bibliographical note

Funding Information:
Supported in part by grant lPO1 HL36552 from the National Institute of Health and the Kentucky Agricultural Experiment Station (article number 94-9 133).


  • Atherosclerosis
  • Endothelial cells
  • Oxidized lipids
  • Proteoglycans

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine


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