P2x2 dominant deafness mutations have no negative effect on wild-type isoform: Implications for functional rescue and in deafness mechanism

Yan Zhu, Juline Beudez, Ning Yu, Thomas Grutter, Hong Bo Zhao

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


The P2X2 receptor is an ATP-gated ion channel, assembled by three subunits. Recently, it has been found that heterozygous mutations of P2X2 V60L and G353R can cause autosomal dominant nonsyndromic hearing loss. However, the underlying mechanism remains unclear. The fact that heterozygous mutations cause deafness suggests that the mutations may have dominant-negative effect (DNE) on wild-type (WT) P2X2 isoforms and/or other partners leading to hearing loss. In this study, the effect of these dominant deafness P2X2 mutations on WT P2X2 was investigated. We found that sole transfection of both V60L and G353R deafness mutants could efficiently target to the plasma membrane, like WT P2X2, but exhibit a significantly reduced response to ATP stimulation. Both mutants reduced the channel conductance, but G353R mutation also altered the voltage dependency. Co-expression with WT P2X2 could restore the response to ATP. As the ratio of WT P2X2 vs. mutants increased, the response to ATP was also increased. Computer modeling confirmed that both V60L and G353R dominant-deafness mutant subunits do not have any negative effect on WT P2X2 subunit, when assembled as a heterotrimer. Improper docking or defective gating is the more likely mechanism for impaired channel function by these P2X2 deafness mutations. These results suggest that P2X2 dominant deafness mutations do not have negative effects on WT P2X2 isoforms, and that adding additional WT P2X2 could rescue the lost channel function caused by the deafness mutations. These P2X2 dominant deafness mutations may have negative-effects on other partners leading to hearing loss.

Original languageEnglish
Article number371
JournalFrontiers in Molecular Neuroscience
StatePublished - Nov 13 2017

Bibliographical note

Publisher Copyright:
© 2017 Zhu, Beudez, Yu, Grutter and Zhao.


  • ATP
  • Deafness
  • Dominant negative effect
  • Functional restoration
  • Mutation
  • P2X2 receptor

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience


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