p53-Mediated negative regulation of stathmin/Op18 expression is associated with G2/M cell-cycle arrest

John Inge Johnsen, Oscar N. Aurelio, Zeenat Kwaja, Gunn E. Jörgensen, Natalia S. Pellegata, Rina Plattner, Eric J. Stanbridge, Jean François Cajot

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

Utilizing the technique of differential display of mRNA, we have identified p53-responsive genes that are transcriptionally up- or down-regulated as cells enter growth arrest. One gene that was down-regulated, pong16, was found to be identical to stathmin/Op18, a protein involved in the regulation of microtubule dynamics. Evidence that p53 is directly or indirectly involved in negative regulation of stathmin/Op18 expression includes the following: (i) p53-mediated growth inhibition is associated with repression of stathmin/Op18 expression following serum stimulation, (ii) reporter gene assays revealed p53-mediated repression of stathmin/Op18 promoter activity and (iii) constitutive over-expression of stathmin/Op18 bypasses a p53-mediated G2/M arrest in the cell cycle. These results suggest that p53-mediated negative regulation of stathmin/Op18 plays an important role in cell-cycle control. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)685-691
Number of pages7
JournalInternational Journal of Cancer
Volume88
Issue number5
DOIs
StatePublished - Dec 1 2000

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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