Abstract
Chemical investigation of the endophyte Pseudofusicoccum stromaticum CMRP4328 isolated from the medicinal plant Stryphnodendron adstringens yielded ten compounds, including two new dihydrochromones, paecilins Q (1) and R (2). The antifungal activity of the isolated metabolites was assessed against an important citrus pathogen, Phyllosticta citricarpa. Cytochalasin H (6) (78.3%), phomoxanthone A (3) (70.2%), phomoxanthone B (4) (63.1%), and paecilin Q (1) (50.5%) decreased in vitro the number of pycnidia produced by P. citricarpa, which are responsible for the disease dissemination in orchards. In addition, compounds 3 and 6 inhibited the development of citrus black spot symptoms in citrus fruits. Cytochalasin H (6) and one of the new compounds, paecilin Q (1), appear particularly promising, as they showed strong activity against this citrus pathogen, and low or no cytotoxic activity. The strain CMRP4328 of P. stromaticum and its metabolites deserve further investigation for the control of citrus black spot disease.
| Original language | English |
|---|---|
| Pages (from-to) | 1178-1189 |
| Number of pages | 12 |
| Journal | Planta Medica |
| Volume | 89 |
| Issue number | 12 |
| DOIs | |
| State | Published - Nov 1 2022 |
Bibliographical note
Publisher Copyright:© 2022 Georg Thieme Verlag. All rights reserved.
Funding
Acknowledgements This work was supported by the INCT Citrus CNPq 465440/2014-2 Brazil and CNPq grant 309971/2016-0 and 424738/2016-3 to C.G., CAPES-Brazil - grant to J.I. This work was also supported by National Institutes of Health grants R01 CA243529, R37 AI52218, the Center of Biomedical Research Excellence (COBRE) in Pharmaceutical Research and Innovation (CPRI, NIH P20 GM130456), the University Professorship in Pharmacy (to J.R. and J.S.T.), the University of Kentucky College of Pharmacy, the University of Kentucky Markey Cancer Center, and the National Center for Advancing Translational Sciences (UL1TR000117 and UL1TR001998). We thank the College of Pharmacy NMR Center (University of Kentucky) for NMR support. J.I. thanks the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for a scholarship.
| Funders | Funder number |
|---|---|
| University of Kentucky | |
| University of Kentucky Markey Comprehensive Cancer Center | |
| Center of Biomedical Research Excellence | |
| Coordenação de Aperfeiçoamento de Pessoal de Nível Superior | |
| European Commission | |
| Seventh Framework Programme | 243529 |
| National Center for Advancing Translational Sciences (NCATS) | UL1TR001998, UL1TR000117 |
| National Institutes of Health (NIH) | P20 GM130456, R01 CA243529, R37 AI52218 |
| Conselho Nacional de Desenvolvimento Científico e Tecnológico | 309971/2016-0, 424738/2016-3 |
Keywords
- Phyllosticta citricarpa
- Pseudofusicoccum stromaticum
- Stryphnodendron adstringens
- dihydrochromones
- fungal metabolites
- phytopathogenic fungi
ASJC Scopus subject areas
- Analytical Chemistry
- Molecular Medicine
- Pharmacology
- Pharmaceutical Science
- Drug Discovery
- Complementary and alternative medicine
- Organic Chemistry