Paired Medicago receptors mediate broad-spectrum resistance to nodulation by Sinorhizobium meliloti carrying a species-specific gene

Jinge Liu, Ting Wang, Qiulin Qin, Xiaocheng Yu, Shengming Yang, Randy D. Dinkins, Anett Kuczmog, Péter Putnoky, Artur Muszyński, Joel S. Griffitts, Attila Kereszt, Hongyan Zhu

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Plants have evolved the ability to distinguish between symbiotic and pathogenic microbial signals. However, potentially cooperative plant–microbe interactions often abort due to incompatible signaling. The Nodulation Specificity 1 (NS1) locus in the legume Medicago truncatula blocks tissue invasion and root nodule induction by many strains of the nitrogen-fixing symbiont Sinorhizobium meliloti. Controlling this strain-specific nodulation blockade are two genes at the NS1 locus, designated NS1a and NS1b, which encode malectin-like leucine-rich repeat receptor kinases. Expression of NS1a and NS1b is induced upon inoculation by both compatible and incompatible Sinorhizobium strains and is dependent on host perception of bacterial nodulation (Nod) factors. Both presence/absence and sequence polymorphisms of the paired receptors contribute to the evolution and functional diversification of the NS1 locus. A bacterial gene, designated rns1, is required for activation of NS1-mediated nodulation restriction. rns1 encodes a type I-secreted protein and is present in approximately 50% of the nearly 250 sequenced S. meliloti strains but not found in over 60 sequenced strains from the closely related species Sinorhizobium medicae. S. meliloti strains lacking functional rns1 are able to evade NS1-mediated nodulation blockade.

Original languageEnglish
Article numbere2214703119
JournalProceedings of the National Academy of Sciences of the United States of America
Volume119
Issue number51
DOIs
StatePublished - Dec 20 2022

Bibliographical note

Publisher Copyright:
Copyright © 2022 the Author(s). Published by PNAS.

Keywords

  • Medicago
  • nodulation
  • receptors
  • specificity
  • symbiosis

ASJC Scopus subject areas

  • General

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