Palmitoylation of acetylated tubulin and association with ceramide-rich platforms is critical for ciliogenesis

Priyanka Tripathi, Zhihui Zhu, Haiyan Qin, Ahmed Elsherbini, Simone M. Crivelli, Emily Roush, Guanghu Wang, Stefka D. Spassieva, Erhard Bieberich

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Microtubules are polymers composed of αβ-tubulin subunits that provide structure to cells and play a crucial role in in the development and function of neuronal processes and cilia, microtubule-driven extensions of the plasma membrane that have sensory (primary cilia) or motor (motile cilia) functions. To stabilize microtubules in neuronal processes and cilia, α tubulin is modified by the posttranslational addition of an acetyl group, or acetylation. We discovered that acetylated tubulin in microtubules interacts with the membrane sphingolipid, ceramide. However, the molecular mechanism and function of this interaction are not understood. Here, we show that in human induced pluripotent stem cell-derived neurons, ceramide stabilizes microtubules, which indicates a similar function in cilia. Using proximity ligation assays, we detected complex formation of ceramide with acetylated tubulin in Chlamydomonas reinhardtii flagella and cilia of human embryonic kidney (HEK293T) cells, primary cultured mouse astrocytes, and ependymal cells. Using incorporation of palmitic azide and click chemistry-mediated addition of fluorophores, we show that a portion of acetylated tubulin is S-palmitoylated. S-palmitoylated acetylated tubulin is colocalized with ceramide-rich platforms in the ciliary membrane, and it is coimmunoprecipitated with Arl13b, a GTPase that mediates transport of proteins into cilia. Inhibition of S-palmitoylation with 2-bromo palmitic acid or inhibition of ceramide biosynthesis with fumonisin B1 reduces formation of the Arl13b-acetylated tubulin complex and its transport into cilia, concurrent with impairment of ciliogenesis. Together, these data show, for the first time, that ceramide-rich platforms mediate membrane anchoring and interaction of S-palmitoylated proteins that are critical for cilium formation, stabilization, and function.

Original languageEnglish
Article numberRA120001190
JournalJournal of Lipid Research
Volume62
DOIs
StatePublished - 2021

Bibliographical note

Publisher Copyright:
© 2021 American Society for Biochemistry and Molecular Biology Inc.. All rights reserved.

Keywords

  • Acetylation
  • Arl13b
  • Cell biology
  • Ceramide
  • Cilia
  • Lipid rafts
  • Lipids
  • Microtubules
  • Palmitoylation
  • Tubulin

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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