Pancreatic exocrine secretion in steers infused postruminally with casein and cornstarch

C. J. Richards, K. C. Swanson, S. J. Paton, D. L. Harmon, G. B. Huntington

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

Our objective was to evaluate the effect of postruminal protein infusion on pancreatic exocrine secretions. One Holstein, two crossbred, and five Angus steers (305 ± 5 kg) with pancreatic pouch-duodenal reentrant cannulas and abomasal infusion catheters were used in a replicated 4 x 4 Latin square. All steers were abomasally infused with 1,050 g/d of raw cornstarch with treatments of 0, 60, 120, or 180 g/d of sodium casein suspended in water to yield 6,000 g/d of infusate daily. Steers were limit-fed (1.5 x NEm; 12 equal portions daily) a 90% corn silage, 10% supplement diet formulated to contain 12.5% CP. Periods consisted of 3 d of adaptation to infusion, 7 d of full infusion, 1 d of collection, and 7 d of rest. Pancreatic juice was collected in 30-min fractions continuously for 6 h. Total juice secreted and the pH of individual fractions were recorded, a 10% subsample was retained to form a composite sample, and remaining fluid was returned to the duodenum. Juice composite samples were stored (-30°C) until analyzed for total protein and activities of α-amylase, trypsin, and chymotrypsin. Casein infusion linearly increased α-amylase concentration (182 to 271 units/mL; P < 0.02; 17.5 to 24.6 units/mg of protein; P < 0.03) and secretion rate (26,847 to 41,894 units/h; P < 0.01). Total juice secretion (155 g/h), pH of pancreatic juice (8.13), secretion rate of protein (1,536 mg/h), and concentration of protein (10.2 mg/mL) in pancreatic secretions were not affected (P > 0.05) by casein infusion. Similarly, casein infusion did not change (P > 0.05) trypsin and chymotrypsin concentrations (1,379 and 349 units/L or 0.134 and 0.033 units/mg of protein, respectively) or secretion rates (206 and 52 units/h, respectively). Abomasal infusion of protein with starch stimulated a greater pancreatic secretion of α-amylase activity into the intestine than infusion of starch alone.

Original languageEnglish
Pages (from-to)1051-1056
Number of pages6
JournalJournal of Animal Science
Volume81
Issue number4
DOIs
StatePublished - Apr 2003

Keywords

  • Beef Cattle
  • Pancreas
  • Protein Digestion
  • Ruminants
  • Starch Digestion
  • α-Amylase

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

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