Paramyxoviruses from neotropical bats suggest a novel genus and nephrotropism

William Marciel de Souza; Marcilio Jorge Fumagalli; Jean Paul Carrera; Jansen de Araujo; Jedson Ferreira Cardoso; Cristiano de Carvalho; Edison Luiz Durigon; Luzia Helena Queiroz; Nuno Rodrigues Faria; Pablo R. Murcia; Luiz Tadeu Moraes Figueiredo

doi:10.1016/j.meegid.2021.105041

Paramyxoviruses from neotropical bats suggest a novel genus and nephrotropism

William Marciel de Souza, Marcilio Jorge Fumagalli, Jean Paul Carrera, Jansen de Araujo, Jedson Ferreira Cardoso, Cristiano de Carvalho, Edison Luiz Durigon, Luzia Helena Queiroz, Nuno Rodrigues Faria, Pablo R. Murcia, Luiz Tadeu Moraes Figueiredo

Microbiology, Immunology, and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Paramyxoviruses have a broad host range and geographic distribution, including human pathogens transmitted by bats, such as Nipah and Hendra viruses. In this study, we combined high-throughput sequencing and molecular approaches to investigate the presence of paramyxoviruses in neotropical bats (Microchiroptera suborder) in Brazil. We discovered and characterized three novel paramyxoviruses in the kidney tissues of apparently healthy common vampire bats (D. rotundus) and Seba's short-tailed bats (C. perspicillata), which we tentatively named Kanhgág virus (KANV), Boe virus (BOEV), and Guató virus (GUATV). In this study, we classified these viruses as putative species into the Macrojêvirus genus, a newly proposed genus of the Orthoparamyxovirinae subfamily. Using RT-PCR, we detected these viruses in 20.9% (9 out of 43) of bats tested, and viral RNA was detected exclusively in kidney tissues. Attempts to isolate infectious virus were successful for KANV and GUATV. Our results expand the viral diversity, host range, and geographical distribution of the paramyxoviruses.

Original language	English
Article number	105041
Journal	Infection, Genetics and Evolution
Volume	95
DOIs	https://doi.org/10.1016/j.meegid.2021.105041
State	Published - Nov 2021

Bibliographical note

Publisher Copyright:
© 2021

Funding

This work was supported by the Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo, Brazil (Grant number. 13/14929-1, and Scholarships No. 06/00572-0, 15/05778-5; 17/13981-0, 19/24251-9 and 18/09383-3) and by the CADDE/FAPESP Brazil-UK partnership grant (MR/S0195/1 and FAPESP 18/14389-0) (http://caddecentre.org/). NRF is supported by a Wellcome Trust and Royal Society Sir Henry Dale Fellowship (204311/Z/16/Z). PRM is supported by the Medical Research Council of the United Kingdom (MC_UU_12014/9). National Institutes of Health grant R24 AI120942. This work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo , Brazil (Grant number. 13/14929-1 , and Scholarships No. 06/00572-0 , 15/05778-5 ; 17/13981-0 , 19/24251-9 and 18/09383-3 ) and by the CADDE/FAPESP Brazil-UK partnership grant ( MR/S0195/1 and FAPESP 18/14389-0 ) ( http://caddecentre.org/ ). NRF is supported by a Wellcome Trust and Royal Society Sir Henry Dale Fellowship ( 204311/Z/16/Z ). PRM is supported by the Medical Research Council of the United Kingdom ( MC_UU_12014/9 ). National Institutes of Health grant R24 AI120942 .

Funders	Funder number
Wellcome Trust
UK Medical Research Council, Engineering and Physical Sciences Research Council	MR/R015600/1
Medical Research Council of the United Kingdom	MC_UU_12014/9, R24 AI120942
Royal Society Sir Henry Dale Fellowship	204311/Z/16/Z
Fundação de Amparo à Pesquisa do Estado de São Paulo	13/14929-1, 17/13981-0, 18/09383-3, 18/14389-0, MR/S0195/1, 15/05778-5, 06/00572-0, 19/24251-9

Keywords

Bat-borne diseases
Paramyxoviridae
Paramyxovirus
Virus discovery

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics
Molecular Biology
Genetics
Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.meegid.2021.105041

Cite this

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title = "Paramyxoviruses from neotropical bats suggest a novel genus and nephrotropism",

abstract = "Paramyxoviruses have a broad host range and geographic distribution, including human pathogens transmitted by bats, such as Nipah and Hendra viruses. In this study, we combined high-throughput sequencing and molecular approaches to investigate the presence of paramyxoviruses in neotropical bats (Microchiroptera suborder) in Brazil. We discovered and characterized three novel paramyxoviruses in the kidney tissues of apparently healthy common vampire bats (D. rotundus) and Seba's short-tailed bats (C. perspicillata), which we tentatively named Kanhg{\'a}g virus (KANV), Boe virus (BOEV), and Guat{\'o} virus (GUATV). In this study, we classified these viruses as putative species into the Macroj{\^e}virus genus, a newly proposed genus of the Orthoparamyxovirinae subfamily. Using RT-PCR, we detected these viruses in 20.9\% (9 out of 43) of bats tested, and viral RNA was detected exclusively in kidney tissues. Attempts to isolate infectious virus were successful for KANV and GUATV. Our results expand the viral diversity, host range, and geographical distribution of the paramyxoviruses.",

keywords = "Bat-borne diseases, Paramyxoviridae, Paramyxovirus, Virus discovery",

author = "\{de Souza\}, \{William Marciel\} and Fumagalli, \{Marcilio Jorge\} and Carrera, \{Jean Paul\} and \{de Araujo\}, Jansen and Cardoso, \{Jedson Ferreira\} and \{de Carvalho\}, Cristiano and Durigon, \{Edison Luiz\} and Queiroz, \{Luzia Helena\} and Faria, \{Nuno Rodrigues\} and Murcia, \{Pablo R.\} and Figueiredo, \{Luiz Tadeu Moraes\}",

note = "Publisher Copyright: {\textcopyright} 2021",

year = "2021",

month = nov,

doi = "10.1016/j.meegid.2021.105041",

language = "English",

volume = "95",

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AU - Fumagalli, Marcilio Jorge

AU - Carrera, Jean Paul

AU - de Araujo, Jansen

AU - Cardoso, Jedson Ferreira

AU - de Carvalho, Cristiano

AU - Durigon, Edison Luiz

AU - Queiroz, Luzia Helena

AU - Faria, Nuno Rodrigues

AU - Murcia, Pablo R.

AU - Figueiredo, Luiz Tadeu Moraes

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Paramyxoviruses from neotropical bats suggest a novel genus and nephrotropism

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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