TY - JOUR
T1 - Paternally expressed retrotransposon Gag-like 1 gene, RTL1, is one of the crucial elements for placental angiogenesis in horses
AU - Dini, Pouya
AU - Carossino, Mariano
AU - Balasuriya, Udeni B.R.
AU - El-Sheikh Ali, Hossam
AU - Loux, Shavahn C.
AU - Esteller-Vico, Alejandro
AU - Scoggin, Kirsten E.
AU - Loynachan, Alan T.
AU - Kalbfleisch, Theodore
AU - De Spiegelaere, Ward
AU - Daels, Peter
AU - Ball, Barry A.
N1 - Publisher Copyright:
© 2021 The Author(s) 2021. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: [email protected].
PY - 2021/6/1
Y1 - 2021/6/1
N2 - RTL1 (retrotransposon Gag-like 1) is an essential gene in the development of the human and murine placenta. Several fetal and placental abnormalities such as intrauterine growth restriction (IUGR) and hydrops conditions have been associated with altered expression of this gene. However, the function of RTL1 has not been identified. RTL1 is located on a highly conserved region in eutherian mammals. Therefore, the genetic and molecular analysis in horses could hold important implications for other species, including humans. Here, we demonstrated that RTL1 is paternally expressed and is localized within the endothelial cells of the equine (Equus caballus) chorioallantois. We developed an equine placental microvasculature primary cell culture and demonstrated that RTL1 knockdown leads to loss of the sprouting ability of these endothelial cells. We further demonstrated an association between abnormal expression of RTL1 and development of hydrallantois. Our data suggest that RTL1 may be essential for placental angiogenesis, and its abnormal expression can lead to placental insufficiency. This placental insufficiency could be the reason for IUGR and hydrops conditions reported in other species, including humans.
AB - RTL1 (retrotransposon Gag-like 1) is an essential gene in the development of the human and murine placenta. Several fetal and placental abnormalities such as intrauterine growth restriction (IUGR) and hydrops conditions have been associated with altered expression of this gene. However, the function of RTL1 has not been identified. RTL1 is located on a highly conserved region in eutherian mammals. Therefore, the genetic and molecular analysis in horses could hold important implications for other species, including humans. Here, we demonstrated that RTL1 is paternally expressed and is localized within the endothelial cells of the equine (Equus caballus) chorioallantois. We developed an equine placental microvasculature primary cell culture and demonstrated that RTL1 knockdown leads to loss of the sprouting ability of these endothelial cells. We further demonstrated an association between abnormal expression of RTL1 and development of hydrallantois. Our data suggest that RTL1 may be essential for placental angiogenesis, and its abnormal expression can lead to placental insufficiency. This placental insufficiency could be the reason for IUGR and hydrops conditions reported in other species, including humans.
KW - IUGR
KW - RTL1
KW - angiogenesis
KW - chorioallantois
KW - hydrops
KW - paternally expressed gene
KW - placenta
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U2 - 10.1093/biolre/ioab039
DO - 10.1093/biolre/ioab039
M3 - Article
C2 - 33693478
AN - SCOPUS:85107902613
SN - 0006-3363
VL - 104
SP - 1386
EP - 1399
JO - Biology of Reproduction
JF - Biology of Reproduction
IS - 6
ER -