Patterns of salivary analytes provide diagnostic capacity for distinguishing chronic adult periodontitis from health

Jeffrey L. Ebersole; Julie L. Schuster; Jason Stevens; Dolph Dawson; Richard J. Kryscio; Yushin Lin; Mark V. Thomas; Craig S. Miller

doi:10.1007/s10875-012-9771-3

Patterns of salivary analytes provide diagnostic capacity for distinguishing chronic adult periodontitis from health

Jeffrey L. Ebersole, Julie L. Schuster, Jason Stevens, Dolph Dawson, Richard J. Kryscio, Yushin Lin, Mark V. Thomas, Craig S. Miller

Research output: Contribution to journal › Article › peer-review

119 Scopus citations

Abstract

Salivary biomarker discovery requires identification of analytes with high discriminatory capacity to distinguish disease from health, including day-to-day variations that occur in analyte levels. In this study, seven biomarkers associated with inflammatory and tissue destructive processes of periodontal disease were investigated. In a prospective cohort study design, analyte expression levels were determined in unstimulated whole saliva samples collected on multiple occasions from 30 healthy adults (i.e., orally and systemically) and 50 chronic adult periodontitis patients. Salivary levels of IL-1β, IL-6, MMP-8, and albumin were significantly elevated (5.4 to 12.6X) and levels of IFNa were consistently lower (8.7X) in periodontitis patients compared with the daily variation observed in healthy adults. ROC analyses of IL-1β, IL-6 and MMP-8 yielded areas under the curves of 0.963-0.984 for discriminating periodontitis from health. These results demonstrate that levels of salivary bioanalytes of patients who have periodontitis are uniquely different from normal levels found in healthy subjects, and a panel consisting of IL-1β, MMP-8 and IL-6 shows particular diagnostic potential.

Original language	English
Pages (from-to)	271-279
Number of pages	9
Journal	Journal of Clinical Immunology
Volume	33
Issue number	1
DOIs	https://doi.org/10.1007/s10875-012-9771-3
State	Published - Jan 2013

Bibliographical note

Funding Information:
Research Funding This study was supported by grant U01 DE017793, from the NIH/NIDCR, P20 RR020145 from the NIH/ NCRR, and funds from the Center for Oral Health Research in the University of Kentucky College of Dentistry. The authors state no conflict of interest related to this study.

Funding

Research Funding This study was supported by grant U01 DE017793, from the NIH/NIDCR, P20 RR020145 from the NIH/ NCRR, and funds from the Center for Oral Health Research in the University of Kentucky College of Dentistry. The authors state no conflict of interest related to this study.

Funders	Funder number
NIH/NIDCR	P20 RR020145
National Institutes of Health (NIH)
National Center for Research Resources	P20RR020145
University of Kentucky College of Dentistry

Keywords

Analytes
Diagnosis
Inflammation
Periodontitis
Saliva

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1007/s10875-012-9771-3

Cite this

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title = "Patterns of salivary analytes provide diagnostic capacity for distinguishing chronic adult periodontitis from health",

abstract = "Salivary biomarker discovery requires identification of analytes with high discriminatory capacity to distinguish disease from health, including day-to-day variations that occur in analyte levels. In this study, seven biomarkers associated with inflammatory and tissue destructive processes of periodontal disease were investigated. In a prospective cohort study design, analyte expression levels were determined in unstimulated whole saliva samples collected on multiple occasions from 30 healthy adults (i.e., orally and systemically) and 50 chronic adult periodontitis patients. Salivary levels of IL-1β, IL-6, MMP-8, and albumin were significantly elevated (5.4 to 12.6X) and levels of IFNa were consistently lower (8.7X) in periodontitis patients compared with the daily variation observed in healthy adults. ROC analyses of IL-1β, IL-6 and MMP-8 yielded areas under the curves of 0.963-0.984 for discriminating periodontitis from health. These results demonstrate that levels of salivary bioanalytes of patients who have periodontitis are uniquely different from normal levels found in healthy subjects, and a panel consisting of IL-1β, MMP-8 and IL-6 shows particular diagnostic potential.",

keywords = "Analytes, Diagnosis, Inflammation, Periodontitis, Saliva",

author = "Ebersole, {Jeffrey L.} and Schuster, {Julie L.} and Jason Stevens and Dolph Dawson and Kryscio, {Richard J.} and Yushin Lin and Thomas, {Mark V.} and Miller, {Craig S.}",

note = "Funding Information: Research Funding This study was supported by grant U01 DE017793, from the NIH/NIDCR, P20 RR020145 from the NIH/ NCRR, and funds from the Center for Oral Health Research in the University of Kentucky College of Dentistry. The authors state no conflict of interest related to this study.",

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AU - Lin, Yushin

AU - Thomas, Mark V.

AU - Miller, Craig S.

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N2 - Salivary biomarker discovery requires identification of analytes with high discriminatory capacity to distinguish disease from health, including day-to-day variations that occur in analyte levels. In this study, seven biomarkers associated with inflammatory and tissue destructive processes of periodontal disease were investigated. In a prospective cohort study design, analyte expression levels were determined in unstimulated whole saliva samples collected on multiple occasions from 30 healthy adults (i.e., orally and systemically) and 50 chronic adult periodontitis patients. Salivary levels of IL-1β, IL-6, MMP-8, and albumin were significantly elevated (5.4 to 12.6X) and levels of IFNa were consistently lower (8.7X) in periodontitis patients compared with the daily variation observed in healthy adults. ROC analyses of IL-1β, IL-6 and MMP-8 yielded areas under the curves of 0.963-0.984 for discriminating periodontitis from health. These results demonstrate that levels of salivary bioanalytes of patients who have periodontitis are uniquely different from normal levels found in healthy subjects, and a panel consisting of IL-1β, MMP-8 and IL-6 shows particular diagnostic potential.

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Patterns of salivary analytes provide diagnostic capacity for distinguishing chronic adult periodontitis from health

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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