Persistent organic pollutants such as polychlorinated biphenyls (PCBs) are associated with detrimental health outcomes including cardiovascular diseases. Remediation of these compounds is a critical component of environmental policy. Although remediation efforts aim to completely remove toxicants, little is known about the effects of potential remediation byproducts. We previously published that Fe/Pd nanoparticles effectively dechlorinate PCB 77 to biphenyl, thus eliminating PCB-induced endothelial dysfunction using primary vascular endothelial cells. Herein, we analyzed the toxic effects of PCB congener mixtures (representative mixtures of commercial PCBs based on previous dechlorination data) produced at multiple time points during the dechlorination of PCB 77 to biphenyl. Compared with pure PCB 77, exposing endothelial cells to lower chlorinated PCB byproducts led to improved cellular viability, decreased superoxide production, and decreased nuclear factor kappa B activation based on duration of remediation. Presence of the parent compound, PCB 77, led to significant increases in mRNA and protein inflammatory marker expression. These data implicate that PCB dechlorination reduces biological toxicity to vascular endothelial cells.
|Number of pages||11|
|Journal||Environmental Science and Pollution Research|
|State||Published - May 2014|
Bibliographical noteFunding Information:
Acknowledgments This work was supported by National Institutes of Health/National Institute of Environmental Health Sciences grant P42ES007380, American Recovery and Reinvestment Act (ARRA) funds (3P42ES007380-13S1), and with funds from the University of Kentucky Agricultural Experiment Station. We would like to thank Christopher R. Barton1 and Alex N. Palumbo1 for their contributions to the preliminary work on this project. Thanks also to Dr. Seong-Su Han for his work with the NFкB binding assay. A special thanks to Michael Petriello for his editorial assistance.
- Endothelial cell dysfunction
- Oxidative stress
- Pollutant remediation
ASJC Scopus subject areas
- Environmental Chemistry
- Health, Toxicology and Mutagenesis