Peptide conjugated magnetic nanoparticles for magnetically mediated energy delivery to lung cancer cells

Anastasia K. Hauser, Kimberly W. Anderson, J. Zach Hilt

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Aim: In the present study, we examine the effects of internalized peptide-conjugated iron oxide nanoparticles and their ability to locally convert alternating magnetic field (AMF) energy into other forms of energy (e.g., heat and rotational work). Materials & methods: Dextran-coated iron oxide nanoparticles were functionalized with a cell penetrating peptide and after internalization by A549 and H358 cells were activated by an AMF. Results: TAT-functionalized nanoparticles and AMF exposure increased reactive oxygen species generation compared with the nanoparticle system alone. The TAT-functionalized nanoparticles induced lysosomal membrane permeability and mitochondrial membrane depolarization, but these effects were not further enhanced by AMF treatment. Although not statistically significant, there are trends suggesting an increase in apoptosis via the Caspase 3/7 pathways when cells are exposed to TAT-functionalized nanoparticles combined with AMF. Conclusion: Our results indicate that internalized TAT-functionalized iron oxide nanoparticles activated by an AMF elicit cellular responses without a measurable temperature rise.

Original languageEnglish
Pages (from-to)1769-1785
Number of pages17
JournalNanomedicine
Volume11
Issue number14
DOIs
StatePublished - Jul 2016

Bibliographical note

Publisher Copyright:
© 2016 Future Medicine Ltd.

Keywords

  • TAT
  • cell penetrating peptide
  • iron oxide nanoparticles
  • magnetically medicated energy delivery
  • reactive oxygen species

ASJC Scopus subject areas

  • Bioengineering
  • Development
  • Materials Science (all)
  • Biomedical Engineering
  • Medicine (miscellaneous)

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