Peptide fractionation and free radical scavenging activity of zein hydrolysate

Xueyan Tang; Zhiyong He; Yanfeng Dai; Youling L. Xiong; Mingyong Xie; Jie Chen

doi:10.1021/jf9028656

Peptide fractionation and free radical scavenging activity of zein hydrolysate

Xueyan Tang, Zhiyong He, Yanfeng Dai, Youling L. Xiong, Mingyong Xie, Jie Chen

Animal and Food Sciences

Research output: Contribution to journal › Article › peer-review

199 Scopus citations

Abstract

Alcalase-treated zein hydrolysate (ZH) was separated by gel filtration, ultrafiltration, and reversedphase HPLC, and the scavenging activities for 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH'), and superoxide anion (O₂) radicals of different peptide fractions were measured to establish the antioxidant efficacy. Results showed that the ability to stabilize water-soluble free radicals (ABTS) by ZH components was insensitive to the peptide size, whereas that against ethanol-soluble free radicals (DPPH) and O₂ was molecular weight dependent. Antioxidative peptides of <1 kDa were further separated by HPLC into 30 components, of which those with great hydrophobicity exhibited strong DPPH and O₂ scavenging ability and those with intermediate hydrophobicity displayed the maximum ABTS scavenging activity. Two dominant components (fractions 8 and 17) were further purified and identified by LC-PDA-ESI-MS to be Tyr-Ala and Leu-Met-Cys-His, respectively. The results demonstrated that the free radical scavenging activity of ZH depended on the radical species and was strongly related to the molecular weight and hydrophobicity of the constituting peptides.

Original language	English
Pages (from-to)	587-593
Number of pages	7
Journal	Journal of Agricultural and Food Chemistry
Volume	58
Issue number	1
DOIs	https://doi.org/10.1021/jf9028656
State	Published - Jan 13 2010

Keywords

Antioxidant
Free radical scavenging
Gel flltration
Peptides fractionation
Zein hydrolysate

ASJC Scopus subject areas

General Chemistry
General Agricultural and Biological Sciences

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10.1021/jf9028656

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title = "Peptide fractionation and free radical scavenging activity of zein hydrolysate",

abstract = "Alcalase-treated zein hydrolysate (ZH) was separated by gel filtration, ultrafiltration, and reversedphase HPLC, and the scavenging activities for 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH'), and superoxide anion (O2) radicals of different peptide fractions were measured to establish the antioxidant efficacy. Results showed that the ability to stabilize water-soluble free radicals (ABTS) by ZH components was insensitive to the peptide size, whereas that against ethanol-soluble free radicals (DPPH) and O2 was molecular weight dependent. Antioxidative peptides of <1 kDa were further separated by HPLC into 30 components, of which those with great hydrophobicity exhibited strong DPPH and O2 scavenging ability and those with intermediate hydrophobicity displayed the maximum ABTS scavenging activity. Two dominant components (fractions 8 and 17) were further purified and identified by LC-PDA-ESI-MS to be Tyr-Ala and Leu-Met-Cys-His, respectively. The results demonstrated that the free radical scavenging activity of ZH depended on the radical species and was strongly related to the molecular weight and hydrophobicity of the constituting peptides.",

keywords = "Antioxidant, Free radical scavenging, Gel flltration, Peptides fractionation, Zein hydrolysate",

author = "Xueyan Tang and Zhiyong He and Yanfeng Dai and Xiong, {Youling L.} and Mingyong Xie and Jie Chen",

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AU - Tang, Xueyan

AU - He, Zhiyong

AU - Dai, Yanfeng

AU - Xiong, Youling L.

AU - Xie, Mingyong

AU - Chen, Jie

PY - 2010/1/13

Y1 - 2010/1/13

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AB - Alcalase-treated zein hydrolysate (ZH) was separated by gel filtration, ultrafiltration, and reversedphase HPLC, and the scavenging activities for 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH'), and superoxide anion (O2) radicals of different peptide fractions were measured to establish the antioxidant efficacy. Results showed that the ability to stabilize water-soluble free radicals (ABTS) by ZH components was insensitive to the peptide size, whereas that against ethanol-soluble free radicals (DPPH) and O2 was molecular weight dependent. Antioxidative peptides of <1 kDa were further separated by HPLC into 30 components, of which those with great hydrophobicity exhibited strong DPPH and O2 scavenging ability and those with intermediate hydrophobicity displayed the maximum ABTS scavenging activity. Two dominant components (fractions 8 and 17) were further purified and identified by LC-PDA-ESI-MS to be Tyr-Ala and Leu-Met-Cys-His, respectively. The results demonstrated that the free radical scavenging activity of ZH depended on the radical species and was strongly related to the molecular weight and hydrophobicity of the constituting peptides.

KW - Antioxidant

KW - Free radical scavenging

KW - Gel flltration

KW - Peptides fractionation

KW - Zein hydrolysate

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Peptide fractionation and free radical scavenging activity of zein hydrolysate

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