Peptide fractionation and free radical scavenging activity of zein hydrolysate

Xueyan Tang, Zhiyong He, Yanfeng Dai, Youling L. Xiong, Mingyong Xie, Jie Chen

Research output: Contribution to journalArticlepeer-review

191 Scopus citations


Alcalase-treated zein hydrolysate (ZH) was separated by gel filtration, ultrafiltration, and reversedphase HPLC, and the scavenging activities for 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH'), and superoxide anion (O2) radicals of different peptide fractions were measured to establish the antioxidant efficacy. Results showed that the ability to stabilize water-soluble free radicals (ABTS) by ZH components was insensitive to the peptide size, whereas that against ethanol-soluble free radicals (DPPH) and O2 was molecular weight dependent. Antioxidative peptides of <1 kDa were further separated by HPLC into 30 components, of which those with great hydrophobicity exhibited strong DPPH and O2 scavenging ability and those with intermediate hydrophobicity displayed the maximum ABTS scavenging activity. Two dominant components (fractions 8 and 17) were further purified and identified by LC-PDA-ESI-MS to be Tyr-Ala and Leu-Met-Cys-His, respectively. The results demonstrated that the free radical scavenging activity of ZH depended on the radical species and was strongly related to the molecular weight and hydrophobicity of the constituting peptides.

Original languageEnglish
Pages (from-to)587-593
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Issue number1
StatePublished - Jan 13 2010


  • Antioxidant
  • Free radical scavenging
  • Gel flltration
  • Peptides fractionation
  • Zein hydrolysate

ASJC Scopus subject areas

  • General Chemistry
  • General Agricultural and Biological Sciences


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