Performance of ePlex® blood culture identification panels in clinical isolates and characterization of antimicrobial stewardship opportunities

Jenni K. Thomas; Justin Clark; Vaneet Arora; David S. Burgess; Donna R. Burgess; Ryan P. Mynatt; Jeremy D. VanHoose; Katie L. Wallace; Sarah E. Cotner

doi:10.1016/j.diagmicrobio.2024.116269

Performance of ePlex® blood culture identification panels in clinical isolates and characterization of antimicrobial stewardship opportunities

Jenni K. Thomas
, Justin Clark
, Vaneet Arora
, David S. Burgess
, Donna R. Burgess
, Ryan P. Mynatt
, Jeremy D. VanHoose
, Katie L. Wallace
, Sarah E. Cotner

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We assessed the performance of GenMark's ePlex® Blood Culture Identification (BCID) Panels for overall agreement of organism identification and resistance mechanism detection with standard microbiologic methods. This study included patients with a positive blood culture from May 2020 to January 2021. The primary outcomes were to assess concordance of ePlex® organism identification with standard identification methods and concordance of ePlex® genotypic resistance mechanism detection with standard phenotypic susceptibility testing. Secondary outcomes included panel specific performance and characterization of antimicrobial stewardship opportunities. The overall identification concordance rate in 1276 positive blood cultures was 98.1%. The overall concordance for the presence of resistance markers was 98.2% and concordance for the absence of resistance markers was 100%. A majority of ePlex® results (69.5%) represented opportunities for potential antimicrobial stewardship intervention. High concordance rates between the ePlex® BCID panels and standard identification and susceptibility methods enable utilization of results to guide rapid antimicrobial optimization.

Original language	English
Article number	116269
Journal	Diagnostic Microbiology and Infectious Disease
Volume	109
Issue number	3
DOIs	https://doi.org/10.1016/j.diagmicrobio.2024.116269
State	Published - Jul 2024

Bibliographical note

Publisher Copyright:
© 2024

Funding

The project described was supported by the NIH National Center for Advancing Translational Sciences through grant number UL1TR001998. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.

Funders	Funder number
National Institutes of Health (NIH)
National Center for Advancing Translational Sciences (NCATS)	UL1TR001998
National Center for Advancing Translational Sciences (NCATS)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Antimicrobial stewardship
Bacteremia
Bacterial identification
Blood cultures
ePlex®
Rapid diagnostics

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

Access to Document

10.1016/j.diagmicrobio.2024.116269

Cite this

Thomas, J. K., Clark, J., Arora, V., Burgess, D. S., Burgess, D. R., Mynatt, R. P., VanHoose, J. D., Wallace, K. L., & Cotner, S. E. (2024). Performance of ePlex® blood culture identification panels in clinical isolates and characterization of antimicrobial stewardship opportunities. Diagnostic Microbiology and Infectious Disease, 109(3), Article 116269. https://doi.org/10.1016/j.diagmicrobio.2024.116269

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title = "Performance of ePlex{\textregistered} blood culture identification panels in clinical isolates and characterization of antimicrobial stewardship opportunities",

abstract = "We assessed the performance of GenMark's ePlex{\textregistered} Blood Culture Identification (BCID) Panels for overall agreement of organism identification and resistance mechanism detection with standard microbiologic methods. This study included patients with a positive blood culture from May 2020 to January 2021. The primary outcomes were to assess concordance of ePlex{\textregistered} organism identification with standard identification methods and concordance of ePlex{\textregistered} genotypic resistance mechanism detection with standard phenotypic susceptibility testing. Secondary outcomes included panel specific performance and characterization of antimicrobial stewardship opportunities. The overall identification concordance rate in 1276 positive blood cultures was 98.1\%. The overall concordance for the presence of resistance markers was 98.2\% and concordance for the absence of resistance markers was 100\%. A majority of ePlex{\textregistered} results (69.5\%) represented opportunities for potential antimicrobial stewardship intervention. High concordance rates between the ePlex{\textregistered} BCID panels and standard identification and susceptibility methods enable utilization of results to guide rapid antimicrobial optimization.",

keywords = "Antimicrobial stewardship, Bacteremia, Bacterial identification, Blood cultures, ePlex{\textregistered}, Rapid diagnostics",

author = "Thomas, \{Jenni K.\} and Justin Clark and Vaneet Arora and Burgess, \{David S.\} and Burgess, \{Donna R.\} and Mynatt, \{Ryan P.\} and VanHoose, \{Jeremy D.\} and Wallace, \{Katie L.\} and Cotner, \{Sarah E.\}",

note = "Publisher Copyright: {\textcopyright} 2024",

year = "2024",

month = jul,

doi = "10.1016/j.diagmicrobio.2024.116269",

language = "English",

volume = "109",

number = "3",

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Thomas, JK, Clark, J, Arora, V , Burgess, DS, Burgess, DR, Mynatt, RP, VanHoose, JD, Wallace, KL & Cotner, SE 2024, 'Performance of ePlex® blood culture identification panels in clinical isolates and characterization of antimicrobial stewardship opportunities', Diagnostic Microbiology and Infectious Disease, vol. 109, no. 3, 116269. https://doi.org/10.1016/j.diagmicrobio.2024.116269

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AU - Thomas, Jenni K.

AU - Clark, Justin

AU - Arora, Vaneet

AU - Burgess, David S.

AU - Burgess, Donna R.

AU - Mynatt, Ryan P.

AU - VanHoose, Jeremy D.

AU - Wallace, Katie L.

AU - Cotner, Sarah E.

PY - 2024/7

Y1 - 2024/7

N2 - We assessed the performance of GenMark's ePlex® Blood Culture Identification (BCID) Panels for overall agreement of organism identification and resistance mechanism detection with standard microbiologic methods. This study included patients with a positive blood culture from May 2020 to January 2021. The primary outcomes were to assess concordance of ePlex® organism identification with standard identification methods and concordance of ePlex® genotypic resistance mechanism detection with standard phenotypic susceptibility testing. Secondary outcomes included panel specific performance and characterization of antimicrobial stewardship opportunities. The overall identification concordance rate in 1276 positive blood cultures was 98.1%. The overall concordance for the presence of resistance markers was 98.2% and concordance for the absence of resistance markers was 100%. A majority of ePlex® results (69.5%) represented opportunities for potential antimicrobial stewardship intervention. High concordance rates between the ePlex® BCID panels and standard identification and susceptibility methods enable utilization of results to guide rapid antimicrobial optimization.

AB - We assessed the performance of GenMark's ePlex® Blood Culture Identification (BCID) Panels for overall agreement of organism identification and resistance mechanism detection with standard microbiologic methods. This study included patients with a positive blood culture from May 2020 to January 2021. The primary outcomes were to assess concordance of ePlex® organism identification with standard identification methods and concordance of ePlex® genotypic resistance mechanism detection with standard phenotypic susceptibility testing. Secondary outcomes included panel specific performance and characterization of antimicrobial stewardship opportunities. The overall identification concordance rate in 1276 positive blood cultures was 98.1%. The overall concordance for the presence of resistance markers was 98.2% and concordance for the absence of resistance markers was 100%. A majority of ePlex® results (69.5%) represented opportunities for potential antimicrobial stewardship intervention. High concordance rates between the ePlex® BCID panels and standard identification and susceptibility methods enable utilization of results to guide rapid antimicrobial optimization.

KW - Antimicrobial stewardship

KW - Bacteremia

KW - Bacterial identification

KW - Blood cultures

KW - ePlex®

KW - Rapid diagnostics

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JO - Diagnostic Microbiology and Infectious Disease

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Performance of ePlex® blood culture identification panels in clinical isolates and characterization of antimicrobial stewardship opportunities

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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Cite this