Periodicity in the quantity and blend ratios of pheromone components in glands and volatile emissions of mutant and normal cabbage looper moths, Trichoplusia ni

Randy E. Hunt; Kenneth F. Haynes

doi:10.1016/0022-1910(90)90050-P

Periodicity in the quantity and blend ratios of pheromone components in glands and volatile emissions of mutant and normal cabbage looper moths, Trichoplusia ni

Randy E. Hunt, Kenneth F. Haynes

Entomology

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42 Scopus citations

Abstract

Pheromone release behaviour, pheromone content of glands, and volatile emission rates of pheromone from female Trichoplusia ni were determined during the 24 h photoperiodic regime. Periodic changes in the quantity and blend ratios of pheromone emitted from forcibly extruded glands were similar for normal females and females that express a mutant gene for an abnormal pheromone phenotype. Both types of females exhibited a diel periodicity in the emission rate and gland content of (Z)-7-dodecenyl acetate (Z7-12:Ac), the major component. Emission rates of this compound were low during the photophase, rose rapidly at the beginning of the scotophase, and then decreased later in the scotophase. In contrast, the amount of Z7-12:Ac in glands increased during the photophase and then decreased throughout the scotophase. Periodicity in the amount of 12-carbon acetate minor components in volatile emissions and glands followed the same pattern as Z7-12:Ac, thus their relative proportions (proportions relative to Z7-12:Ac) remained constant during light and dark periods. In contrast, the relative proportion of the 14-carbon acetate components in volatile emissions rose during the scotophase and then decreased during the photophase. Our results suggest that: (1) normal and mutant females are similar in terms of photoperiodic regulation of pheromone biosynthesis, pheromone release behaviour, and pheromone emission despite the differences in the pheromone blend of the two types, (2) pheromone biosynthesis is continuous, (3) periodicity in emissions is a manifestation of an underlying periodicity in transport of pheromone to the gland's surface that is correlated with pheromone release behaviour but not pheromone biosynthesis, and (4) pheromone blend ratios change during the course of the pheromone release period because of different vapour pressures of the pheromone components.

Original language	English
Pages (from-to)	769-774
Number of pages	6
Journal	Journal of Insect Physiology
Volume	36
Issue number	10
DOIs	https://doi.org/10.1016/0022-1910(90)90050-P
State	Published - 1990

Bibliographical note

Funding Information:
Acknowledgements-We thankW endellR oelofs for helpful suggestiondsu ringt he courseo f this study.T his materiali s based upon work supported by the Cooperative State ResearchS ervice,U .S. Departmento f Agriculture,u nder AgreementN os 87-CRCR-1-2410a nd 89-37153-435W4.e acknowledget he support of the University of Kentucky Major ResearchI nstrumentationB ond Program in the purchaseo f equipmentu sedi n this study (ID#s 7E-8164-11, L-250-SAOSOL, -251-8A080a nd L-252-SAOSO)T. his investigation(p aperN o. 90-7-49w) asc onductedin connection with a projecto f theK entuckyA griculturalS tationa nd is publishedw ith the approvalo f the Director.

Keywords

Diel periodicity
Lepidoptera
Noctuidae
Trichoplusia ni
pheromone release behaviour
sex pheromone

ASJC Scopus subject areas

Physiology
Insect Science

Access to Document

10.1016/0022-1910(90)90050-P

Cite this

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title = "Periodicity in the quantity and blend ratios of pheromone components in glands and volatile emissions of mutant and normal cabbage looper moths, Trichoplusia ni",

abstract = "Pheromone release behaviour, pheromone content of glands, and volatile emission rates of pheromone from female Trichoplusia ni were determined during the 24 h photoperiodic regime. Periodic changes in the quantity and blend ratios of pheromone emitted from forcibly extruded glands were similar for normal females and females that express a mutant gene for an abnormal pheromone phenotype. Both types of females exhibited a diel periodicity in the emission rate and gland content of (Z)-7-dodecenyl acetate (Z7-12:Ac), the major component. Emission rates of this compound were low during the photophase, rose rapidly at the beginning of the scotophase, and then decreased later in the scotophase. In contrast, the amount of Z7-12:Ac in glands increased during the photophase and then decreased throughout the scotophase. Periodicity in the amount of 12-carbon acetate minor components in volatile emissions and glands followed the same pattern as Z7-12:Ac, thus their relative proportions (proportions relative to Z7-12:Ac) remained constant during light and dark periods. In contrast, the relative proportion of the 14-carbon acetate components in volatile emissions rose during the scotophase and then decreased during the photophase. Our results suggest that: (1) normal and mutant females are similar in terms of photoperiodic regulation of pheromone biosynthesis, pheromone release behaviour, and pheromone emission despite the differences in the pheromone blend of the two types, (2) pheromone biosynthesis is continuous, (3) periodicity in emissions is a manifestation of an underlying periodicity in transport of pheromone to the gland's surface that is correlated with pheromone release behaviour but not pheromone biosynthesis, and (4) pheromone blend ratios change during the course of the pheromone release period because of different vapour pressures of the pheromone components.",

keywords = "Diel periodicity, Lepidoptera, Noctuidae, Trichoplusia ni, pheromone release behaviour, sex pheromone",

author = "Hunt, {Randy E.} and Haynes, {Kenneth F.}",

note = "Funding Information: Acknowledgements-We thankW endellR oelofs for helpful suggestiondsu ringt he courseo f this study.T his materiali s based upon work supported by the Cooperative State ResearchS ervice,U .S. Departmento f Agriculture,u nder AgreementN os 87-CRCR-1-2410a nd 89-37153-435W4.e acknowledget he support of the University of Kentucky Major ResearchI nstrumentationB ond Program in the purchaseo f equipmentu sedi n this study (ID#s 7E-8164-11, L-250-SAOSOL, -251-8A080a nd L-252-SAOSO)T. his investigation(p aperN o. 90-7-49w) asc onductedin connection with a projecto f theK entuckyA griculturalS tationa nd is publishedw ith the approvalo f the Director.",

year = "1990",

doi = "10.1016/0022-1910(90)90050-P",

language = "English",

volume = "36",

pages = "769--774",

number = "10",

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TY - JOUR

T1 - Periodicity in the quantity and blend ratios of pheromone components in glands and volatile emissions of mutant and normal cabbage looper moths, Trichoplusia ni

AU - Hunt, Randy E.

AU - Haynes, Kenneth F.

N1 - Funding Information: Acknowledgements-We thankW endellR oelofs for helpful suggestiondsu ringt he courseo f this study.T his materiali s based upon work supported by the Cooperative State ResearchS ervice,U .S. Departmento f Agriculture,u nder AgreementN os 87-CRCR-1-2410a nd 89-37153-435W4.e acknowledget he support of the University of Kentucky Major ResearchI nstrumentationB ond Program in the purchaseo f equipmentu sedi n this study (ID#s 7E-8164-11, L-250-SAOSOL, -251-8A080a nd L-252-SAOSO)T. his investigation(p aperN o. 90-7-49w) asc onductedin connection with a projecto f theK entuckyA griculturalS tationa nd is publishedw ith the approvalo f the Director.

PY - 1990

Y1 - 1990

N2 - Pheromone release behaviour, pheromone content of glands, and volatile emission rates of pheromone from female Trichoplusia ni were determined during the 24 h photoperiodic regime. Periodic changes in the quantity and blend ratios of pheromone emitted from forcibly extruded glands were similar for normal females and females that express a mutant gene for an abnormal pheromone phenotype. Both types of females exhibited a diel periodicity in the emission rate and gland content of (Z)-7-dodecenyl acetate (Z7-12:Ac), the major component. Emission rates of this compound were low during the photophase, rose rapidly at the beginning of the scotophase, and then decreased later in the scotophase. In contrast, the amount of Z7-12:Ac in glands increased during the photophase and then decreased throughout the scotophase. Periodicity in the amount of 12-carbon acetate minor components in volatile emissions and glands followed the same pattern as Z7-12:Ac, thus their relative proportions (proportions relative to Z7-12:Ac) remained constant during light and dark periods. In contrast, the relative proportion of the 14-carbon acetate components in volatile emissions rose during the scotophase and then decreased during the photophase. Our results suggest that: (1) normal and mutant females are similar in terms of photoperiodic regulation of pheromone biosynthesis, pheromone release behaviour, and pheromone emission despite the differences in the pheromone blend of the two types, (2) pheromone biosynthesis is continuous, (3) periodicity in emissions is a manifestation of an underlying periodicity in transport of pheromone to the gland's surface that is correlated with pheromone release behaviour but not pheromone biosynthesis, and (4) pheromone blend ratios change during the course of the pheromone release period because of different vapour pressures of the pheromone components.

AB - Pheromone release behaviour, pheromone content of glands, and volatile emission rates of pheromone from female Trichoplusia ni were determined during the 24 h photoperiodic regime. Periodic changes in the quantity and blend ratios of pheromone emitted from forcibly extruded glands were similar for normal females and females that express a mutant gene for an abnormal pheromone phenotype. Both types of females exhibited a diel periodicity in the emission rate and gland content of (Z)-7-dodecenyl acetate (Z7-12:Ac), the major component. Emission rates of this compound were low during the photophase, rose rapidly at the beginning of the scotophase, and then decreased later in the scotophase. In contrast, the amount of Z7-12:Ac in glands increased during the photophase and then decreased throughout the scotophase. Periodicity in the amount of 12-carbon acetate minor components in volatile emissions and glands followed the same pattern as Z7-12:Ac, thus their relative proportions (proportions relative to Z7-12:Ac) remained constant during light and dark periods. In contrast, the relative proportion of the 14-carbon acetate components in volatile emissions rose during the scotophase and then decreased during the photophase. Our results suggest that: (1) normal and mutant females are similar in terms of photoperiodic regulation of pheromone biosynthesis, pheromone release behaviour, and pheromone emission despite the differences in the pheromone blend of the two types, (2) pheromone biosynthesis is continuous, (3) periodicity in emissions is a manifestation of an underlying periodicity in transport of pheromone to the gland's surface that is correlated with pheromone release behaviour but not pheromone biosynthesis, and (4) pheromone blend ratios change during the course of the pheromone release period because of different vapour pressures of the pheromone components.

KW - Diel periodicity

KW - Lepidoptera

KW - Noctuidae

KW - Trichoplusia ni

KW - pheromone release behaviour

KW - sex pheromone

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SN - 0022-1910

VL - 36

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Periodicity in the quantity and blend ratios of pheromone components in glands and volatile emissions of mutant and normal cabbage looper moths, Trichoplusia ni

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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