Periodontal treatment reduces matrix metalloproteinase levels in localized aggressive periodontitis

Patricia Furtado Goncxalves, Hong Huang, Suzanna McAninley, Barnett Alfant, Peter Harrison, Ikramuddin Aukhil, Clay Walker, Luciana Macchion Shaddox

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


Background: Matrix metalloproteinases (MMPs) are a family of host-derived proteinases reported to mediate multiple functions associated with periodontal breakdown and inflammation. High MMP levels in African-American children with localized aggressive periodontitis (LAgP) have been reported previously by the present authors. However, little is known about MMP reductions in gingival crevicular fluid (GCF) after therapy. This study aims to evaluate MMP levels in the GCF after treatment of LAgP and to correlate these levels with clinical response. Methods: GCF samples were collected from 29 African- American individuals diagnosed with LAgP. GCF was collected from one diseased site (probing depth [PD] >4 mm, bleeding on probing [BOP], and clinical attachment level ≥2 mm) and one healthy site (PD ≤3 mm, no BOP) from each individual at baseline and 3 and 6 months after periodontal treatment, which consisted of full-mouth scaling and root planing (SRP) and systemic antibiotics. The volume of GCF was controlled using a calibrated gingival fluid meter, and levels of MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-12, and MMP-13 were assessed using fluorometric kits. Results: MMP-1, MMP-8, MMP-9, MMP-12, and MMP-13 levels were reduced significantly up to 6 months, comparable to healthy sites at the same point. Significant correlations were noted between MMP-2, MMP-3, MMP-8, MMP-9, MMP-12, and MMP-13 levels and percentage of sites with PD >4 mm. MMP-3, MMP-12, and MMP-13 levels also correlated with mean PD of affected sites. Conclusion: Treatment of LAgP with SRP and systemic antibiotics was effective in reducing local levels of specific MMPs in African-American individuals, which correlated positively with some clinical parameters.

Original languageEnglish
Pages (from-to)1801-1808
Number of pages8
JournalJournal of Periodontology
Issue number12
StatePublished - Dec 2013


  • Adolescent
  • Aggressive periodontitis
  • Anti-infective agents
  • Child
  • Metalloproteinases
  • Root planing

ASJC Scopus subject areas

  • Periodontics


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