TY - JOUR
T1 - Periostin regulation and cartilage degradation early after anterior cruciate ligament reconstruction
AU - Jacobs, Cale A.
AU - Keller, Laura E.
AU - Zhang, Sheng
AU - Fu, Qin
AU - Hunt, Emily R.
AU - Stone, Austin V.
AU - Conley, Caitlin E.W.
AU - Lattermann, Christian
AU - Fortier, Lisa A.
N1 - Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature Switzerland AG.
PY - 2023/3
Y1 - 2023/3
N2 - Objective and design: The purpose of this study was to explore pathological processes during the first 4 weeks after anterior cruciate ligament reconstruction (ACLR). Subjects: Sixteen ACL-injured patients (8 females/8 males, mean age = 19.1, mean BMI = 28.6). Methods: Arthrocentesis was performed 1 and 4 weeks after ACLR. Proteins in the synovial fluid were identified using nanoLC–ESI-MS/MS. Differentially up- or down-regulated proteins were identified and quantified, and a pathway analysis was performed. All identified proteins were mapped into a protein–protein interaction (PPI) network, and networks of PPIs with a combined score > 0.9 were then visualized. Results: Seven pathways were upregulated after ACLR: PI3K-AKT signaling pathway, extracellular matrix (ECM)–receptor interaction, focal adhesion, protein digestion and absorption, ameobiasis, and platelet activation. Network analyses identified 8 proteins that were differentially upregulated with strong PPI interactions (periostin and 7 collagen-related proteins). Increases in periostin moderately correlated with increases in a synovial fluid biomarker of type II cartilage degradation (ρ = 0.51, p = 0.06). Conclusion: Pro-inflammatory pathways and periostin were upregulated after ACLR. Periostin demonstrated strong network connections with markers of collagen breakdown, and future work is needed to determine whether periostin may offer a biomarker of early cartilage degradation after ACLR and/or play an active role in early post-traumatic osteoarthritis (PTOA) progression.
AB - Objective and design: The purpose of this study was to explore pathological processes during the first 4 weeks after anterior cruciate ligament reconstruction (ACLR). Subjects: Sixteen ACL-injured patients (8 females/8 males, mean age = 19.1, mean BMI = 28.6). Methods: Arthrocentesis was performed 1 and 4 weeks after ACLR. Proteins in the synovial fluid were identified using nanoLC–ESI-MS/MS. Differentially up- or down-regulated proteins were identified and quantified, and a pathway analysis was performed. All identified proteins were mapped into a protein–protein interaction (PPI) network, and networks of PPIs with a combined score > 0.9 were then visualized. Results: Seven pathways were upregulated after ACLR: PI3K-AKT signaling pathway, extracellular matrix (ECM)–receptor interaction, focal adhesion, protein digestion and absorption, ameobiasis, and platelet activation. Network analyses identified 8 proteins that were differentially upregulated with strong PPI interactions (periostin and 7 collagen-related proteins). Increases in periostin moderately correlated with increases in a synovial fluid biomarker of type II cartilage degradation (ρ = 0.51, p = 0.06). Conclusion: Pro-inflammatory pathways and periostin were upregulated after ACLR. Periostin demonstrated strong network connections with markers of collagen breakdown, and future work is needed to determine whether periostin may offer a biomarker of early cartilage degradation after ACLR and/or play an active role in early post-traumatic osteoarthritis (PTOA) progression.
KW - Biomarker
KW - Cartilage
KW - Knee
KW - Network analysis
KW - Pathway analysis
KW - Synovial fluid
UR - http://www.scopus.com/inward/record.url?scp=85144737395&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85144737395&partnerID=8YFLogxK
U2 - 10.1007/s00011-022-01678-9
DO - 10.1007/s00011-022-01678-9
M3 - Article
C2 - 36562795
AN - SCOPUS:85144737395
SN - 1023-3830
VL - 72
SP - 387
EP - 394
JO - Inflammation Research
JF - Inflammation Research
IS - 3
ER -