Periostin regulation and cartilage degradation early after anterior cruciate ligament reconstruction

Cale A. Jacobs, Laura E. Keller, Sheng Zhang, Qin Fu, Emily R. Hunt, Austin V. Stone, Caitlin E.W. Conley, Christian Lattermann, Lisa A. Fortier

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Objective and design: The purpose of this study was to explore pathological processes during the first 4 weeks after anterior cruciate ligament reconstruction (ACLR). Subjects: Sixteen ACL-injured patients (8 females/8 males, mean age = 19.1, mean BMI = 28.6). Methods: Arthrocentesis was performed 1 and 4 weeks after ACLR. Proteins in the synovial fluid were identified using nanoLC–ESI-MS/MS. Differentially up- or down-regulated proteins were identified and quantified, and a pathway analysis was performed. All identified proteins were mapped into a protein–protein interaction (PPI) network, and networks of PPIs with a combined score > 0.9 were then visualized. Results: Seven pathways were upregulated after ACLR: PI3K-AKT signaling pathway, extracellular matrix (ECM)–receptor interaction, focal adhesion, protein digestion and absorption, ameobiasis, and platelet activation. Network analyses identified 8 proteins that were differentially upregulated with strong PPI interactions (periostin and 7 collagen-related proteins). Increases in periostin moderately correlated with increases in a synovial fluid biomarker of type II cartilage degradation (ρ = 0.51, p = 0.06). Conclusion: Pro-inflammatory pathways and periostin were upregulated after ACLR. Periostin demonstrated strong network connections with markers of collagen breakdown, and future work is needed to determine whether periostin may offer a biomarker of early cartilage degradation after ACLR and/or play an active role in early post-traumatic osteoarthritis (PTOA) progression.

Original languageEnglish
Pages (from-to)387-394
Number of pages8
JournalInflammation Research
Issue number3
StatePublished - Mar 2023

Bibliographical note

Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature Switzerland AG.


  • Biomarker
  • Cartilage
  • Knee
  • Network analysis
  • Pathway analysis
  • Synovial fluid

ASJC Scopus subject areas

  • Immunology
  • Pharmacology


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