Phaeophleospora vochysiae Savi & Glienke sp. nov. Isolated from Vochysia divergens Found in the Pantanal, Brazil, Produces Bioactive Secondary Metabolites

Daiani C. Savi; Khaled A. Shaaban; Francielly Maria Wilke Ramos Gos; Larissa V. Ponomareva; Jon S. Thorson; Chirlei Glienke; Jürgen Rohr

doi:10.1038/s41598-018-21400-2

Phaeophleospora vochysiae Savi & Glienke sp. nov. Isolated from Vochysia divergens Found in the Pantanal, Brazil, Produces Bioactive Secondary Metabolites

Daiani C. Savi, Khaled A. Shaaban, Francielly Maria Wilke Ramos Gos, Larissa V. Ponomareva, Jon S. Thorson, Chirlei Glienke, Jürgen Rohr

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Abstract

Microorganisms associated with plants are highly diverse and can produce a large number of secondary metabolites, with antimicrobial, anti-parasitic and cytotoxic activities. We are particularly interested in exploring endophytes from medicinal plants found in the Pantanal, a unique and widely unexplored wetland in Brazil. In a bio-prospecting study, strains LGMF1213 and LGMF1215 were isolated as endophytes from Vochysia divergens, and by morphological and molecular phylogenetic analyses were characterized as Phaeophleospora vochysiae sp. nov. The chemical assessment of this species reveals three major compounds with high biological activity, cercoscosporin (1), isocercosporin (2) and the new compound 3-(sec-butyl)-6-ethyl-4,5-dihydroxy-2-methoxy-6-methylcyclohex-2-enone (3). Besides the isolation of P. vochysiae as endophyte, the production of cercosporin compounds suggest that under specific conditions this species causes leaf spots, and may turn into a pathogen, since leaf spots are commonly caused by species of Cercospora that produce related compounds. In addition, the new compound 3-(sec-butyl)-6-ethyl-4,5-dihydroxy-2-methoxy-6-methylcyclohex-2-enone showed considerable antimicrobial activity and low cytotoxicity, which needs further exploration.

Original language	English
Article number	3122
Journal	Scientific Reports
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41598-018-21400-2
State	Published - Dec 1 2018

Bibliographical note

Funding Information:
This work was supported by NIH grants CA 91091, GM 105977 and an Endowed University Professorship in Pharmacy to J.R. This work was also supported by the University of Kentucky Markey Cancer Center, the National Center for Advancing Translational Sciences (UL1TR001998) and NIH grants R01 GM115261 to J. S. T. Additional support came from Fundação Araucária de Apoio e Desenvolvimento Científico e Tecnológico do Paraná – Brazil, grant 441/2012–23510, CNPq-Brazil grant 486016/2011–0 to C.G., and CAPES-Brazil – a grant to D.C.S. We also thank Lisandra S. F. Maba and the Laboratório de Microscopia de Fluorescência Convencional e Confocal, UFPR, for image acquisitions.

Publisher Copyright:
© 2018 The Author(s).

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title = "Phaeophleospora vochysiae Savi & Glienke sp. nov. Isolated from Vochysia divergens Found in the Pantanal, Brazil, Produces Bioactive Secondary Metabolites",

abstract = "Microorganisms associated with plants are highly diverse and can produce a large number of secondary metabolites, with antimicrobial, anti-parasitic and cytotoxic activities. We are particularly interested in exploring endophytes from medicinal plants found in the Pantanal, a unique and widely unexplored wetland in Brazil. In a bio-prospecting study, strains LGMF1213 and LGMF1215 were isolated as endophytes from Vochysia divergens, and by morphological and molecular phylogenetic analyses were characterized as Phaeophleospora vochysiae sp. nov. The chemical assessment of this species reveals three major compounds with high biological activity, cercoscosporin (1), isocercosporin (2) and the new compound 3-(sec-butyl)-6-ethyl-4,5-dihydroxy-2-methoxy-6-methylcyclohex-2-enone (3). Besides the isolation of P. vochysiae as endophyte, the production of cercosporin compounds suggest that under specific conditions this species causes leaf spots, and may turn into a pathogen, since leaf spots are commonly caused by species of Cercospora that produce related compounds. In addition, the new compound 3-(sec-butyl)-6-ethyl-4,5-dihydroxy-2-methoxy-6-methylcyclohex-2-enone showed considerable antimicrobial activity and low cytotoxicity, which needs further exploration.",

author = "Savi, {Daiani C.} and Shaaban, {Khaled A.} and Gos, {Francielly Maria Wilke Ramos} and Ponomareva, {Larissa V.} and Thorson, {Jon S.} and Chirlei Glienke and J{\"u}rgen Rohr",

note = "Funding Information: This work was supported by NIH grants CA 91091, GM 105977 and an Endowed University Professorship in Pharmacy to J.R. This work was also supported by the University of Kentucky Markey Cancer Center, the National Center for Advancing Translational Sciences (UL1TR001998) and NIH grants R01 GM115261 to J. S. T. Additional support came from Funda{\c c}{\~a}o Arauc{\'a}ria de Apoio e Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico do Paran{\'a} – Brazil, grant 441/2012–23510, CNPq-Brazil grant 486016/2011–0 to C.G., and CAPES-Brazil – a grant to D.C.S. We also thank Lisandra S. F. Maba and the Laborat{\'o}rio de Microscopia de Fluoresc{\^e}ncia Convencional e Confocal, UFPR, for image acquisitions. Publisher Copyright: {\textcopyright} 2018 The Author(s).",

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AU - Savi, Daiani C.

AU - Shaaban, Khaled A.

AU - Gos, Francielly Maria Wilke Ramos

AU - Ponomareva, Larissa V.

AU - Thorson, Jon S.

AU - Glienke, Chirlei

AU - Rohr, Jürgen

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Phaeophleospora vochysiae Savi & Glienke sp. nov. Isolated from Vochysia divergens Found in the Pantanal, Brazil, Produces Bioactive Secondary Metabolites

Abstract

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