Phage display library screening for identifi cation of interacting protein partners

Balasubrahmanyam Addepalli, Suryadevara Rao, Arthur G. Hunt

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Phage display is a versatile high-throughput screening method employed to understand and improve the chemical biology, be it production of human monoclonal antibodies or identifi cation of interacting protein partners. A majority of cell proteins operate in a concerted fashion either by stable or transient interactions. Such interactions can be mediated by recognition of small amino acid sequence motifs on the protein surface. Phage display can play a crucial role in identifi cation of such motifs. This report describes the use of phage display for the identifi cation of high affi nity sequence motifs that could be responsible for interactions with a target (bait) protein.

Original languageEnglish
Pages (from-to)147-158
Number of pages12
JournalMethods in Molecular Biology
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.


  • High-throughput screening
  • Phage display
  • Protein–protein interactions
  • Random combinatorial approach

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology


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