Phage display library screening for identification of interacting protein partners

Balasubrahmanyam Addepalli, Suryadevara Rao, Arthur G. Hunt

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Phage display is a versatile high-throughput screening method employed to understand and improve the chemical biology, be it production of human monoclonal antibodies or identification of interacting protein partners. A majority of cell proteins operate in a concerted fashion either by stable or transient interactions. Such interactions can be mediated by recognition of small amino acid sequence motifs on the protein surface. Phage display can play a crucial role in identification of such motifs. This report describes the use of phage display for the identification of high affinity sequence motifs that could be responsible for interactions with a target (bait) protein.

Original languageEnglish
Pages (from-to)147-158
Number of pages12
JournalMethods in Molecular Biology
StatePublished - 2015

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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