Purpose: Retinoblastoma is a childhood cancer of the retina. Clinical trials have shown that local delivery of broad spectrum chemotherapeutic agents is efficacious. Recent studies characterizing the genomic and epigenomic landscape of retinoblastoma identified spleen tyrosine kinase (SYK) as a promising candidate for targeted therapy. The purpose of this study was to conduct preclinical testing of the SYK antagonist R406 to evaluate it as a candidate for retinoblastoma treatment.
Methods: The efficacy of the SYK antagonist R406 delivered locally in a human orthotopic xenograft mouse model of retinoblastoma was tested. Intraocular exposure of R406 was determined for various routes and formulations.
Results: There was no evidence of efficacy for subconjunctival. R406. Maximal vitreal concentration was 10-fold lower than the minimal concentration required to kill retinoblastoma cells in vitro. Dosage of R406 subconjunctivally from emulsion or suspension formulations, direct intravitreal injection of the soluble prodrug of R406 (R788), and repeated topical administration of R406 all increased vitreal exposure, but failed to reach the exposure required for retinoblastoma cell death in culture.
Conclusion: Taken together, these data suggest that R406 is not a viable clinical candidate for the treatment of retinoblastoma. This study highlights the importance of pharmacokinetic testing of molecular targeted retinoblastoma therapeutics.
|Number of pages||13|
|State||Published - Nov 2014|
Bibliographical noteFunding Information:
The authors thank Justin Thurman and Alex Su for help collecting tissue samples. The authors thank Justina McEvoy, Claudia Benavente and Daniel Hiler for providing murine vitreous. The authors thank William Wu and Jianrong Wu in the SJCRH Department of Biostatistics. The authors thank William Caufield for preliminary bioanalytical assay development. We thank the American Lebanese Syrian Associated Charities (ALSAC) and St Jude Children’s Research Hospital for funding. Eleanor Pritchard was funded by an SJCRH Academic Programs special fellowship. This work was also supported by a grant from the National Cancer Institute [R01CA168875-01] and by the Howard Hughes Medical Institute.
© 2014 The Author(s).
- Ocular drug delivery
- Spleen tyrosine kinase
ASJC Scopus subject areas
- Molecular Medicine
- Pharmaceutical Science
- Organic Chemistry
- Pharmacology (medical)