Phenomenological partial-specific volumes for G-quadruplex DNAs

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Accurate partial-specific volume (v̄) values are required for sedimentation velocity and sedimentation equilibrium analyses. For nucleic acids, the estimation of these values is complicated by the fact that v̄ depends on base composition, secondary structure, solvation and the concentrations and identities of ions in the surrounding buffer. Here we describe sedimentation equilibrium measurements of the apparent isopotential partial-specific volume /φ for two G-quadruplex DNAs and a single-stranded DNA of similar molecular weight and base composition. The G-quadruplex DNAs are a 22 nucleotide fragment of the human telomere consensus sequence and a 27 nucleotide fragment from the human c-myc promoter. The singlestranded DNA is 26 nucleotides long and is designed to have low propensity to form secondary structures. Parallel measurements were made in buffers containing NaCl and in buffers containing KCl, spanning the range 0.09 M ≤ [salt] ≥ 2.3 M. Limiting values of /φ, extrapolated to [salt] = 0 M, were: 22-mer (NaCl-form), 0.525 ± 0.004 mL/g; 22-mer (KCl-form), 0.531 ± 0.006 mL/g; 27-mer (NaCl-form), 0.548 ± 0.005 mL/g; 27-mer (KCl-form), 0.557 ± 0.006 mL/g; 26-mer (NaCl-form), 0.555 ± 0.004 mL/g; 26-mer (KCl-form), 0.564 ± 0.006 mL/g. Small changes in /φ with [salt] suggest that large changes in counterion association or hydration are unlikely to take place over these concentration ranges.

Original languageEnglish
Pages (from-to)389-396
Number of pages8
JournalEuropean Biophysics Journal
Issue number3
StatePublished - Feb 2010


  • Analytical ultracentrifugation
  • G-quadruplex DNA
  • Partial-specific volume
  • Single-stranded DNA

ASJC Scopus subject areas

  • Biophysics


Dive into the research topics of 'Phenomenological partial-specific volumes for G-quadruplex DNAs'. Together they form a unique fingerprint.

Cite this