TY - JOUR
T1 - Phosphatidylinositol 3-kinase signaling is involved in neurogenesis during Xenopus embryonic development
AU - Peng, Ying
AU - Jiang, Bing Hua
AU - Yang, Pai Hao
AU - Cao, Zongxian
AU - Shi, Xianglin
AU - Lin, Marie C.M.
AU - He, Ming Liang
AU - Kung, Hsiang Fu
PY - 2004/7/2
Y1 - 2004/7/2
N2 - Phosphatidylinositol 3-kinase (PI3K) has numerous cellular functions, including cell survival and proliferation. In this study, we demonstrated that the expression of the active form of PI3K induced dorsal differentiation and axis duplication and strongly induced the expression of neural markers. In contrast, the inhibition of PI3K activity by its dominant negative mutant induced the phenotype of losing posterior structures and the expression of ventral markers. Akt is an essential target of PI3K for neurogenesis. The expression of the active form of Akt induced axis duplication and increased the expression of neural markers. Inhibition of the Akt activity abolished the PI3K-induced double heads and axes. This signal transmits through its target, glycogen synthase kinase 3β, which is known to mediate Wnt signaling for Xenopus development. These results identify a new function of PI3K/Akt signaling in axis formation and neurogenesis during Xenopus embryonic development and provide a direct link between growth factor-mediated PI3K/Akt signaling and Wnt signaling during embryonic development.
AB - Phosphatidylinositol 3-kinase (PI3K) has numerous cellular functions, including cell survival and proliferation. In this study, we demonstrated that the expression of the active form of PI3K induced dorsal differentiation and axis duplication and strongly induced the expression of neural markers. In contrast, the inhibition of PI3K activity by its dominant negative mutant induced the phenotype of losing posterior structures and the expression of ventral markers. Akt is an essential target of PI3K for neurogenesis. The expression of the active form of Akt induced axis duplication and increased the expression of neural markers. Inhibition of the Akt activity abolished the PI3K-induced double heads and axes. This signal transmits through its target, glycogen synthase kinase 3β, which is known to mediate Wnt signaling for Xenopus development. These results identify a new function of PI3K/Akt signaling in axis formation and neurogenesis during Xenopus embryonic development and provide a direct link between growth factor-mediated PI3K/Akt signaling and Wnt signaling during embryonic development.
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U2 - 10.1074/jbc.M402294200
DO - 10.1074/jbc.M402294200
M3 - Article
C2 - 15123704
AN - SCOPUS:3142579906
SN - 0021-9258
VL - 279
SP - 28509
EP - 28514
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 27
ER -