TY - JOUR
T1 - Phospholipase D signaling is essential for meiosis
AU - Rose, Kristine
AU - Rudge, Simon A.
AU - Frohman, Michael A.
AU - Morris, Andrew J.
AU - Engebrecht, Joanne
PY - 1995/12/19
Y1 - 1995/12/19
N2 - Phospholipid metabolism plays an important role in cellular regulation by generating second messengers for signal transduction. Many stimuli activate a phospholipase D, which catalyzes the hydrolysis of phosphatidylcholine, producing phosphatidic acid and choline. Here we report that the yeast SPO14 gene, which is essential for meiosis [Honigberg, S. M., Conicella, C. and Esposito, R. E. (1992) Genetics 130, 703-716], encodes a phospholipase D. SPO14 RNA and protein activity are induced during late meiotic prophase, and the enzyme has properties similar to mammalian phosphatidylinositol 4,5- bisphosphate-regulated phospholipase D. Characterization of an unusual allele of SPO14 defines regions of the protein important for enzyme catalysis and regulation. These results implicate phospholipase D signaling in regulating cellular differentiation.
AB - Phospholipid metabolism plays an important role in cellular regulation by generating second messengers for signal transduction. Many stimuli activate a phospholipase D, which catalyzes the hydrolysis of phosphatidylcholine, producing phosphatidic acid and choline. Here we report that the yeast SPO14 gene, which is essential for meiosis [Honigberg, S. M., Conicella, C. and Esposito, R. E. (1992) Genetics 130, 703-716], encodes a phospholipase D. SPO14 RNA and protein activity are induced during late meiotic prophase, and the enzyme has properties similar to mammalian phosphatidylinositol 4,5- bisphosphate-regulated phospholipase D. Characterization of an unusual allele of SPO14 defines regions of the protein important for enzyme catalysis and regulation. These results implicate phospholipase D signaling in regulating cellular differentiation.
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U2 - 10.1073/pnas.92.26.12151
DO - 10.1073/pnas.92.26.12151
M3 - Article
C2 - 8618862
AN - SCOPUS:0029584034
SN - 0027-8424
VL - 92
SP - 12151
EP - 12155
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 26
ER -