Phospholipase D1: A key factor for the exocytotic machinery in neuroendocrine cells

Nicolas Vitale, Anne Sophie Caumont, Sylvette Chasserot-Golaz, Guangwei Du, Si Wu, Vicki A. Sciorra, Andrew J. Morris, Michael A. Frohman, Marie France Bader

Research output: Contribution to journalArticlepeer-review

206 Citations (SciVal)

Abstract

Phospholipase D (PLD) has been proposed to mediate cytoskeletal remodeling and vesicular trafficking along the secretory pathway. We recently described the activation of an ADP ribosylation factor-regulated PLD at the plasma membrane of chromaffin cells undergoing secretagogue-stimulated exocytosis. We show here that the isoform involved is PLD1b, and, using a real-time assay for individual cells, that PLD activation and exocytosis are closely correlated. Moreover, overexpressed PLD1, but not PLD2, increases stimulated exocytosis in a phosphatidylinositol 4,5-bisphosphate-dependent manner, whereas catalytically inactive PLD1 inhibits it. These results provide the first direct evidence that PLD1 is an important component of the exocytotic machinery in neuroendocrine cells.

Original languageEnglish
Pages (from-to)2424-2434
Number of pages11
JournalEMBO Journal
Volume20
Issue number10
DOIs
StatePublished - May 15 2001

Keywords

  • Chromaffin cell
  • Cytoskeleton
  • Exocytosis
  • Phospholipase D
  • Secretory granule

ASJC Scopus subject areas

  • Neuroscience (all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology (all)
  • Immunology and Microbiology (all)

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