Phospholipids impact the protective effects of HDL-mimetic nanodiscs against lipopolysaccharide-induced inflammation

Sang Yeop Kim, Jukyung Kang, Maria V. Fawaz, Minzhi Yu, Ziyun Xia, Emily E. Morin, Ling Mei, Karl Olsen, Xiang An Li, Anna Schwendeman

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: The impacts of synthetic high-density lipoprotein (sHDL) phospholipid components on anti-sepsis effects were investigated. Methods: sHDL composed with ApoA-I mimetic peptide (22A) and different phosphatidylcholines were prepared and characterized. Anti-inflammatory effects were investigated in vitro and in vivo on lipopolysaccharide (LPS)-induced inflammation models. Results: sHDLs composed with 1,2-dimyristoyl-sn-glycero-3-phosphocholine (22A-DMPC) most effectively neutralizes LPS, inhibits toll-like receptor 4 recruitment into lipid rafts, suppresses nuclear factor κB signaling and promotes activating transcription factor 3 activating. The lethal endotoxemia animal model showed the protective effects of 22A-DMPC. Conclusion: Phospholipid components affect the stability and fluidity of nanodiscs, impacting the anti-septic efficacy of sHDLs. 22A-DMPC presents the strongest LPS binding and anti-inflammatory effects in vitro and in vivo, suggesting a potential sepsis treatment.

Original languageEnglish
Pages (from-to)2127-2142
Number of pages16
JournalNanomedicine
Volume18
Issue number29
DOIs
StatePublished - Dec 1 2023

Bibliographical note

Publisher Copyright:
© 2024 Expert Publishing Medicine Ltd trading as Taylor & Francis.

Keywords

  • endotoxemia
  • high-density lipoproteins
  • nanodiscs
  • phospholipids
  • sepsis

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • General Materials Science
  • Development

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