Abstract
Aim: The impacts of synthetic high-density lipoprotein (sHDL) phospholipid components on anti-sepsis effects were investigated. Methods: sHDL composed with ApoA-I mimetic peptide (22A) and different phosphatidylcholines were prepared and characterized. Anti-inflammatory effects were investigated in vitro and in vivo on lipopolysaccharide (LPS)-induced inflammation models. Results: sHDLs composed with 1,2-dimyristoyl-sn-glycero-3-phosphocholine (22A-DMPC) most effectively neutralizes LPS, inhibits toll-like receptor 4 recruitment into lipid rafts, suppresses nuclear factor κB signaling and promotes activating transcription factor 3 activating. The lethal endotoxemia animal model showed the protective effects of 22A-DMPC. Conclusion: Phospholipid components affect the stability and fluidity of nanodiscs, impacting the anti-septic efficacy of sHDLs. 22A-DMPC presents the strongest LPS binding and anti-inflammatory effects in vitro and in vivo, suggesting a potential sepsis treatment.
| Original language | English |
|---|---|
| Pages (from-to) | 2127-2142 |
| Number of pages | 16 |
| Journal | Nanomedicine |
| Volume | 18 |
| Issue number | 29 |
| DOIs | |
| State | Published - Dec 1 2023 |
Bibliographical note
Publisher Copyright:© 2024 Expert Publishing Medicine Ltd trading as Taylor & Francis.
Keywords
- endotoxemia
- high-density lipoproteins
- nanodiscs
- phospholipids
- sepsis
ASJC Scopus subject areas
- Bioengineering
- Medicine (miscellaneous)
- Biomedical Engineering
- General Materials Science
- Development