Phospholipids impact the protective effects of HDL-mimetic nanodiscs against lipopolysaccharide-induced inflammation

Sang Yeop Kim; Jukyung Kang; Maria V. Fawaz; Minzhi Yu; Ziyun Xia; Emily E. Morin; Ling Mei; Karl Olsen; Xiang An Li; Anna Schwendeman

doi:10.2217/nnm-2023-0222

Phospholipids impact the protective effects of HDL-mimetic nanodiscs against lipopolysaccharide-induced inflammation

Sang Yeop Kim
, Jukyung Kang
, Maria V. Fawaz
, Minzhi Yu
, Ziyun Xia
, Emily E. Morin
, Ling Mei
, Karl Olsen
, Xiang An Li
, Anna Schwendeman

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Aim: The impacts of synthetic high-density lipoprotein (sHDL) phospholipid components on anti-sepsis effects were investigated. Methods: sHDL composed with ApoA-I mimetic peptide (22A) and different phosphatidylcholines were prepared and characterized. Anti-inflammatory effects were investigated in vitro and in vivo on lipopolysaccharide (LPS)-induced inflammation models. Results: sHDLs composed with 1,2-dimyristoyl-sn-glycero-3-phosphocholine (22A-DMPC) most effectively neutralizes LPS, inhibits toll-like receptor 4 recruitment into lipid rafts, suppresses nuclear factor κB signaling and promotes activating transcription factor 3 activating. The lethal endotoxemia animal model showed the protective effects of 22A-DMPC. Conclusion: Phospholipid components affect the stability and fluidity of nanodiscs, impacting the anti-septic efficacy of sHDLs. 22A-DMPC presents the strongest LPS binding and anti-inflammatory effects in vitro and in vivo, suggesting a potential sepsis treatment.

Original language	English
Pages (from-to)	2127-2142
Number of pages	16
Journal	Nanomedicine
Volume	18
Issue number	29
DOIs	https://doi.org/10.2217/nnm-2023-0222
State	Published - Dec 1 2023

Bibliographical note

Publisher Copyright:
© 2024 Expert Publishing Medicine Ltd trading as Taylor & Francis.

Funding

This publication was funded by R01GM113832 (X-A Li and A Schwendeman), VA I01BX004639 (X-A Li) and R01GM121796 (X-A Li) as well as support from American Heart Association 20POST3521818 (L Mei), NIH T32 GM007767 (MV Fawaz), NIH T32 GM008353 (EE Morin), NIH T32 HL125242 (MV Fawaz and EE Morin). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. Competing interests disclosure

Funders	Funder number
National Institutes of Health (NIH)	T32 GM008353, T32 HL125242, T32 GM007767
National Institutes of Health (NIH)
American the American Heart Association	20POST3521818
American the American Heart Association

Keywords

endotoxemia
high-density lipoproteins
nanodiscs
phospholipids
sepsis

ASJC Scopus subject areas

Bioengineering
Medicine (miscellaneous)
Biomedical Engineering
General Materials Science
Development

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10.2217/nnm-2023-0222

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title = "Phospholipids impact the protective effects of HDL-mimetic nanodiscs against lipopolysaccharide-induced inflammation",

abstract = "Aim: The impacts of synthetic high-density lipoprotein (sHDL) phospholipid components on anti-sepsis effects were investigated. Methods: sHDL composed with ApoA-I mimetic peptide (22A) and different phosphatidylcholines were prepared and characterized. Anti-inflammatory effects were investigated in vitro and in vivo on lipopolysaccharide (LPS)-induced inflammation models. Results: sHDLs composed with 1,2-dimyristoyl-sn-glycero-3-phosphocholine (22A-DMPC) most effectively neutralizes LPS, inhibits toll-like receptor 4 recruitment into lipid rafts, suppresses nuclear factor κB signaling and promotes activating transcription factor 3 activating. The lethal endotoxemia animal model showed the protective effects of 22A-DMPC. Conclusion: Phospholipid components affect the stability and fluidity of nanodiscs, impacting the anti-septic efficacy of sHDLs. 22A-DMPC presents the strongest LPS binding and anti-inflammatory effects in vitro and in vivo, suggesting a potential sepsis treatment.",

keywords = "endotoxemia, high-density lipoproteins, nanodiscs, phospholipids, sepsis",

author = "Kim, \{Sang Yeop\} and Jukyung Kang and Fawaz, \{Maria V.\} and Minzhi Yu and Ziyun Xia and Morin, \{Emily E.\} and Ling Mei and Karl Olsen and Li, \{Xiang An\} and Anna Schwendeman",

note = "Publisher Copyright: {\textcopyright} 2024 Expert Publishing Medicine Ltd trading as Taylor \& Francis.",

year = "2023",

month = dec,

day = "1",

doi = "10.2217/nnm-2023-0222",

language = "English",

volume = "18",

pages = "2127--2142",

number = "29",

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TY - JOUR

T1 - Phospholipids impact the protective effects of HDL-mimetic nanodiscs against lipopolysaccharide-induced inflammation

AU - Kim, Sang Yeop

AU - Kang, Jukyung

AU - Fawaz, Maria V.

AU - Yu, Minzhi

AU - Xia, Ziyun

AU - Morin, Emily E.

AU - Mei, Ling

AU - Olsen, Karl

AU - Li, Xiang An

AU - Schwendeman, Anna

PY - 2023/12/1

Y1 - 2023/12/1

N2 - Aim: The impacts of synthetic high-density lipoprotein (sHDL) phospholipid components on anti-sepsis effects were investigated. Methods: sHDL composed with ApoA-I mimetic peptide (22A) and different phosphatidylcholines were prepared and characterized. Anti-inflammatory effects were investigated in vitro and in vivo on lipopolysaccharide (LPS)-induced inflammation models. Results: sHDLs composed with 1,2-dimyristoyl-sn-glycero-3-phosphocholine (22A-DMPC) most effectively neutralizes LPS, inhibits toll-like receptor 4 recruitment into lipid rafts, suppresses nuclear factor κB signaling and promotes activating transcription factor 3 activating. The lethal endotoxemia animal model showed the protective effects of 22A-DMPC. Conclusion: Phospholipid components affect the stability and fluidity of nanodiscs, impacting the anti-septic efficacy of sHDLs. 22A-DMPC presents the strongest LPS binding and anti-inflammatory effects in vitro and in vivo, suggesting a potential sepsis treatment.

AB - Aim: The impacts of synthetic high-density lipoprotein (sHDL) phospholipid components on anti-sepsis effects were investigated. Methods: sHDL composed with ApoA-I mimetic peptide (22A) and different phosphatidylcholines were prepared and characterized. Anti-inflammatory effects were investigated in vitro and in vivo on lipopolysaccharide (LPS)-induced inflammation models. Results: sHDLs composed with 1,2-dimyristoyl-sn-glycero-3-phosphocholine (22A-DMPC) most effectively neutralizes LPS, inhibits toll-like receptor 4 recruitment into lipid rafts, suppresses nuclear factor κB signaling and promotes activating transcription factor 3 activating. The lethal endotoxemia animal model showed the protective effects of 22A-DMPC. Conclusion: Phospholipid components affect the stability and fluidity of nanodiscs, impacting the anti-septic efficacy of sHDLs. 22A-DMPC presents the strongest LPS binding and anti-inflammatory effects in vitro and in vivo, suggesting a potential sepsis treatment.

KW - endotoxemia

KW - high-density lipoproteins

KW - nanodiscs

KW - phospholipids

KW - sepsis

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UR - https://www.scopus.com/pages/publications/85182956232#tab=citedBy

U2 - 10.2217/nnm-2023-0222

DO - 10.2217/nnm-2023-0222

M3 - Article

C2 - 38197376

AN - SCOPUS:85182956232

SN - 1743-5889

VL - 18

SP - 2127

EP - 2142

JO - Nanomedicine

JF - Nanomedicine

IS - 29

ER -

Phospholipids impact the protective effects of HDL-mimetic nanodiscs against lipopolysaccharide-induced inflammation

Abstract

Bibliographical note

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Keywords

ASJC Scopus subject areas

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