Phosphorylation-dependent regulation of septin dynamics during the cell cycle

Jeroen Dobbelaere, Matthew S. Gentry, Richard L. Hallberg, Yves Barral

Research output: Contribution to journalArticlepeer-review

215 Scopus citations

Abstract

Septins are GTPases involved in cytokinesis. In yeast, they form a ring at the cleavage site. Using FRAP, we show that septins are mobile within the ring at bud emergence and telophase and are immobile during S, G2, and M phases. Immobilization of the septins is dependent on both Cla4, a PAK-like kinase, and Gin4, a septin-dependent kinase that can phosphorylate the septin Shs1/Sep7. Induction of septin ring dynamics in telophase is triggered by the translocation of Rts1, a kinetochore-associated regulatory subunit of PP2A phosphatase, to the bud neck and correlates with Rts1-dependent dephosphorylation of Shs1. In rts1-Δ cells, the actomyosin ring contracts properly but cytokinesis fails. Together our results implicate septins in a late step of cytokinesis and indicate that proper regulation of septin dynamics, possibly through the control of their phosphorylation state, is required for the completion of cytokinesis.

Original languageEnglish
Pages (from-to)345-357
Number of pages13
JournalDevelopmental Cell
Volume4
Issue number3
DOIs
StatePublished - Mar 1 2003

Bibliographical note

Funding Information:
We thank M. Peter and C. Mann for providing strains, plasmids, and useful suggestions on the manuscript; S. Gasser, S. Jensen, N. Bouquin, D. Kellogg, R. Li, F. Luca, and U. Surana for strains and plasmids; and L. Dowse, S. Grava, R. Kroschewski, U. Kutay, C. Lüdeke, and D. Liakopoulos for critical reading of the manuscript. We thank the members of the Hallberg and Barral groups for helpful discussions. We are particularly indebted to K. Tatchell and E. Bi for communicating unpublished results, S. Cotesta (curve fittings), C. Berney and G. Danuser (FRAP Microscopy), and R. Moser and T. Lehman (technical support). J.D. and Y.B. are supported by the Swiss Federal Institute of Technology and a grant from the Swiss National Foundation. M.S.G. and R.L.H. were supported by National Science Foundation grants MCB-9603733 and MCB-0113355 awarded to R.L.H.

ASJC Scopus subject areas

  • Molecular Biology
  • General Biochemistry, Genetics and Molecular Biology
  • Developmental Biology
  • Cell Biology

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