Phosphorylation of CAP-G is required for its chromosomal DNA localization during mitosis

Lynea A. Murphy, Kevin D. Sarge

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Condensin is a 5 subunit complex that plays an important role in the structure of chromosomes during mitosis. It is known that phosphorylation of condensin subunits by cdc2/cyclin B at the beginning of mitosis is important for condensin activity, but the sites of these phosphorylation events have not been identified nor has their role in regulating condensin function. Here we identify two threonine residues in the CAP-G subunit of condensin, threonines 308 and 332, that are targets of cdc2/cyclin B phosphorylation. Mutation of these threonines to alanines results in defects in CAP-G localization with chromosomes during mitosis. These results are the first to identify phosphorylation sites within the condensin complex that regulate condensin localization with chromosomal DNA.

Original languageEnglish
Pages (from-to)1007-1011
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume377
Issue number3
DOIs
StatePublished - Dec 19 2008

Bibliographical note

Funding Information:
This research was supported by NIH grant GM64606 to K.D.S. We thank other members of the laboratory for insightful comments during the course of this work.

Keywords

  • CAP-G
  • Cdc2 phosphorylation
  • Condensin
  • Mitosis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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