Phosphorylation of CAP-G is required for its chromosomal DNA localization during mitosis

Lynea A. Murphy; Kevin D. Sarge

doi:10.1016/j.bbrc.2008.10.114

Phosphorylation of CAP-G is required for its chromosomal DNA localization during mitosis

Lynea A. Murphy, Kevin D. Sarge

Molecular and Cellular Biochemistry

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Condensin is a 5 subunit complex that plays an important role in the structure of chromosomes during mitosis. It is known that phosphorylation of condensin subunits by cdc2/cyclin B at the beginning of mitosis is important for condensin activity, but the sites of these phosphorylation events have not been identified nor has their role in regulating condensin function. Here we identify two threonine residues in the CAP-G subunit of condensin, threonines 308 and 332, that are targets of cdc2/cyclin B phosphorylation. Mutation of these threonines to alanines results in defects in CAP-G localization with chromosomes during mitosis. These results are the first to identify phosphorylation sites within the condensin complex that regulate condensin localization with chromosomal DNA.

Original language	English
Pages (from-to)	1007-1011
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	377
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2008.10.114
State	Published - Dec 19 2008

Bibliographical note

Funding Information:
This research was supported by NIH grant GM64606 to K.D.S. We thank other members of the laboratory for insightful comments during the course of this work.

Keywords

CAP-G
Cdc2 phosphorylation
Condensin
Mitosis

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2008.10.114

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title = "Phosphorylation of CAP-G is required for its chromosomal DNA localization during mitosis",

abstract = "Condensin is a 5 subunit complex that plays an important role in the structure of chromosomes during mitosis. It is known that phosphorylation of condensin subunits by cdc2/cyclin B at the beginning of mitosis is important for condensin activity, but the sites of these phosphorylation events have not been identified nor has their role in regulating condensin function. Here we identify two threonine residues in the CAP-G subunit of condensin, threonines 308 and 332, that are targets of cdc2/cyclin B phosphorylation. Mutation of these threonines to alanines results in defects in CAP-G localization with chromosomes during mitosis. These results are the first to identify phosphorylation sites within the condensin complex that regulate condensin localization with chromosomal DNA.",

keywords = "CAP-G, Cdc2 phosphorylation, Condensin, Mitosis",

author = "Murphy, {Lynea A.} and Sarge, {Kevin D.}",

note = "Funding Information: This research was supported by NIH grant GM64606 to K.D.S. We thank other members of the laboratory for insightful comments during the course of this work.",

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doi = "10.1016/j.bbrc.2008.10.114",

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T1 - Phosphorylation of CAP-G is required for its chromosomal DNA localization during mitosis

AU - Murphy, Lynea A.

AU - Sarge, Kevin D.

N1 - Funding Information: This research was supported by NIH grant GM64606 to K.D.S. We thank other members of the laboratory for insightful comments during the course of this work.

PY - 2008/12/19

Y1 - 2008/12/19

N2 - Condensin is a 5 subunit complex that plays an important role in the structure of chromosomes during mitosis. It is known that phosphorylation of condensin subunits by cdc2/cyclin B at the beginning of mitosis is important for condensin activity, but the sites of these phosphorylation events have not been identified nor has their role in regulating condensin function. Here we identify two threonine residues in the CAP-G subunit of condensin, threonines 308 and 332, that are targets of cdc2/cyclin B phosphorylation. Mutation of these threonines to alanines results in defects in CAP-G localization with chromosomes during mitosis. These results are the first to identify phosphorylation sites within the condensin complex that regulate condensin localization with chromosomal DNA.

AB - Condensin is a 5 subunit complex that plays an important role in the structure of chromosomes during mitosis. It is known that phosphorylation of condensin subunits by cdc2/cyclin B at the beginning of mitosis is important for condensin activity, but the sites of these phosphorylation events have not been identified nor has their role in regulating condensin function. Here we identify two threonine residues in the CAP-G subunit of condensin, threonines 308 and 332, that are targets of cdc2/cyclin B phosphorylation. Mutation of these threonines to alanines results in defects in CAP-G localization with chromosomes during mitosis. These results are the first to identify phosphorylation sites within the condensin complex that regulate condensin localization with chromosomal DNA.

KW - CAP-G

KW - Cdc2 phosphorylation

KW - Condensin

KW - Mitosis

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Phosphorylation of CAP-G is required for its chromosomal DNA localization during mitosis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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