TY - JOUR
T1 - Phosphorylation of the rat vesicular acetylcholine transporter
AU - Cho, Goang Won
AU - Kim, Myung Hee
AU - Chai, Young Gyu
AU - Gilmor, Michelle L.
AU - Levey, Alan I.
AU - Hersh, Louis B.
PY - 2000/6/30
Y1 - 2000/6/30
N2 - Metabolic labeling of a mutant PC12 cell line, A123.7, expressing recombinant rat vesicular acetylcholine transporter (VAChT) with radiolabeled inorganic phosphate was used to demonstrate phosphorylation of the transporter on a serine residue. Mutational analysis was used to demonstrate that serine 480, which is located on the COOH-terminal cytoplasmic tail, is the sole phosphorylation site. Phosphorylation of serine 480 was attributable to the action of protein kinase C. Using a permanently dephosphorylated form of rat VAChT, S480A rVAChT, it was shown that this mutant displays the same kinetics for the transport of acetylcholine and the binding of the inhibitor vesamicol as does the wild type transporter. However, sucrose gradient density centrifugation showed that, unlike wild type VAChT, the S480A mutant did not localize to synaptic vesicles. These results suggest that phosphorylation of serine 480 of VAChT is involved in the trafficking of this transporter.
AB - Metabolic labeling of a mutant PC12 cell line, A123.7, expressing recombinant rat vesicular acetylcholine transporter (VAChT) with radiolabeled inorganic phosphate was used to demonstrate phosphorylation of the transporter on a serine residue. Mutational analysis was used to demonstrate that serine 480, which is located on the COOH-terminal cytoplasmic tail, is the sole phosphorylation site. Phosphorylation of serine 480 was attributable to the action of protein kinase C. Using a permanently dephosphorylated form of rat VAChT, S480A rVAChT, it was shown that this mutant displays the same kinetics for the transport of acetylcholine and the binding of the inhibitor vesamicol as does the wild type transporter. However, sucrose gradient density centrifugation showed that, unlike wild type VAChT, the S480A mutant did not localize to synaptic vesicles. These results suggest that phosphorylation of serine 480 of VAChT is involved in the trafficking of this transporter.
UR - http://www.scopus.com/inward/record.url?scp=0034733726&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034733726&partnerID=8YFLogxK
U2 - 10.1074/jbc.M902174199
DO - 10.1074/jbc.M902174199
M3 - Article
C2 - 10748073
AN - SCOPUS:0034733726
SN - 0021-9258
VL - 275
SP - 19942
EP - 19948
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 26
ER -