Phosphorylation of the rat vesicular acetylcholine transporter

Goang Won Cho, Myung Hee Kim, Young Gyu Chai, Michelle L. Gilmor, Alan I. Levey, Louis B. Hersh

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Metabolic labeling of a mutant PC12 cell line, A123.7, expressing recombinant rat vesicular acetylcholine transporter (VAChT) with radiolabeled inorganic phosphate was used to demonstrate phosphorylation of the transporter on a serine residue. Mutational analysis was used to demonstrate that serine 480, which is located on the COOH-terminal cytoplasmic tail, is the sole phosphorylation site. Phosphorylation of serine 480 was attributable to the action of protein kinase C. Using a permanently dephosphorylated form of rat VAChT, S480A rVAChT, it was shown that this mutant displays the same kinetics for the transport of acetylcholine and the binding of the inhibitor vesamicol as does the wild type transporter. However, sucrose gradient density centrifugation showed that, unlike wild type VAChT, the S480A mutant did not localize to synaptic vesicles. These results suggest that phosphorylation of serine 480 of VAChT is involved in the trafficking of this transporter.

Original languageEnglish
Pages (from-to)19942-19948
Number of pages7
JournalJournal of Biological Chemistry
Issue number26
StatePublished - Jun 30 2000

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Phosphorylation of the rat vesicular acetylcholine transporter'. Together they form a unique fingerprint.

Cite this