Photoaffinity Heterobifunctional Cross-Linking Reagents Based on Azide-Substituted Salicylates

Nobuyuki Imai, Lori D. Dwyer, Tadashi Kometani, Tae Ji, Thomas C. Vanaman, David S. Watt

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Photoaffinity heterobifunctional cross-linking reagents are described that incorporate a 4-azidosali-cylate group at one terminus and an N-hydroxysuccinimidyl ester at the other terminus with a “linking arm” of variable length separating the photoactive and electrophilic termini. Exposure of calmodulin (CaM) to succinimidyl N-[2-[(4-azidosalicyloyl)oxy]ethyl]suberamate (4C) led to monoad-ducts at Lys-21, Lys-75, and Lys-94. Separation of the monoadducts from CaM and polyadducts, radioiodination, and photolysis in the presence of human erythrocyte plasma membrane Ca2+, Mg2+-ATPase led to calcium-dependent cross-linking with 8% cross-linking efficiency.

Original languageEnglish
Pages (from-to)144-148
Number of pages5
JournalBioconjugate Chemistry
Volume1
Issue number2
DOIs
StatePublished - Mar 1 1990

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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