TY - JOUR
T1 - Photochemical generation of nitric oxide from nitro-containing compounds
T2 - Possible relation to vascular photorelaxation phenomena
AU - Bauer, John Anthony
AU - Fung, Ho Leung
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1994
Y1 - 1994
N2 - We examined the production of nitric oxide (NO) from various nitro-containing compounds (500 μM and 100 μM solutions in Krebs buffer, pH 7.4). Sealed vials containing solutions of NaNO2, N-nitro-L-arginine 4-nitrophenol, BAY K 8644, or N-nitro-L-arginine methyl ester were stored in the dark, under normal roomlight, or were exposed to ultraviolet light (365 nm), for 30 min (24°C). NO was measured in the vial headspace after 30 min, using a sensitive assay previously established in our laboratory. Production of NO was found to be dependent on the intensity of light exposure for all compounds, and the highest degree of light-induced production of NO was found for NaNO2 and BAY K 8644 solutions. Since NO is a relaxant of smooth muscle, these results help explain the increased sensitivity to relaxation by UV light of vascular and other types of smooth muscle in the presence of NaNO2, BAY K 8644 and N-nitro-L-arginine, as observed by other investigators.
AB - We examined the production of nitric oxide (NO) from various nitro-containing compounds (500 μM and 100 μM solutions in Krebs buffer, pH 7.4). Sealed vials containing solutions of NaNO2, N-nitro-L-arginine 4-nitrophenol, BAY K 8644, or N-nitro-L-arginine methyl ester were stored in the dark, under normal roomlight, or were exposed to ultraviolet light (365 nm), for 30 min (24°C). NO was measured in the vial headspace after 30 min, using a sensitive assay previously established in our laboratory. Production of NO was found to be dependent on the intensity of light exposure for all compounds, and the highest degree of light-induced production of NO was found for NaNO2 and BAY K 8644 solutions. Since NO is a relaxant of smooth muscle, these results help explain the increased sensitivity to relaxation by UV light of vascular and other types of smooth muscle in the presence of NaNO2, BAY K 8644 and N-nitro-L-arginine, as observed by other investigators.
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U2 - 10.1016/0024-3205(94)00578-8
DO - 10.1016/0024-3205(94)00578-8
M3 - Article
C2 - 7504775
AN - SCOPUS:0028366275
SN - 0024-3205
VL - 54
SP - PL1-PL4
JO - Life Sciences
JF - Life Sciences
IS - 1
ER -