Plant peroxisomes function collaboratively with other subcellular organelles, such as chloroplasts and mitochondria, in several metabolic processes. To comprehensively investigate the impact of peroxisomal function on photosynthesis, especially under conditions that are more relevant to natural environments, a systematic screen of over 150 Arabidopsis mutants of genes encoding peroxisomal proteins was conducted using the automated Dynamic Environment Photosynthesis Imager (DEPI). Dynamic and high-light (HL) conditions triggered significant photosynthetic defects in a subset of the mutants, including those of photorespiration (PR) and other peroxisomal processes, some of which may also be related to PR. Further analysis of the PR mutants revealed activation of cyclic electron flow (CEF) around photosystem I and higher accumulation of hydrogen peroxide (H 2 O 2 ) under HL conditions. We hypothesize that impaired PR disturbs the balance of ATP and NADPH, leading to the accumulation of H 2 O 2 that activates CEF to produce ATP to compensate for the imbalance of reducing equivalents. The identification of peroxisomal mutants involved in PR and other peroxisomal functions in the photometric screen will enable further investigation of regulatory links between photosynthesis and PR and interorganellar interaction at the mechanistic level.
|Number of pages||15|
|State||Published - Feb 1 2019|
Bibliographical noteFunding Information:
The authors thank Linda Savage and David Hall for help with DEPI experiments and image processing, Oliver Tessmer for assisting in using OLIVER software, Dr Mio Sato-Cruz and Megan Mulheron for assisting with the Amplex Red assays, Drs Geoffry Davis and Nick Fischer for insightful discussions, Drs Rob Last and Jun Liu for technical assistance and comments on the manuscript, Arabidopsis Biological Resource Center (ABRC) for mutant seeds, Prof. Andreas Weber for the plgg1-1 seeds, and Center for Advanced Algal and Plant Phenotyping (CAAPP) at MSU Plant Research Laboratories for technical support. This work was supported by the Chemical Sciences, Geosciences and Biosciences Division, Office of Basic Energy Sciences, Office of Science, US Department of Energy (DE-FG02-91ER20021) to DMK and JH.
© 2018 The Authors The Plant Journal © 2018 John Wiley & Sons Ltd
- cyclic electron flow
- dynamic environment photosynthesis imager
- dynamic light
- hydrogen peroxide
ASJC Scopus subject areas
- Plant Science
- Cell Biology