Phylogenomics reveals ancient gene tree discordance in the amphibian tree of life

Paul M. Hime, Alan R. Lemmon, Emily C. Moriarty Lemmon, Elizabeth Prendini, Jeremy M. Brown, Robert C. Thomson, Justin D. Kratovil, Brice P. Noonan, R. Alexander Pyron, Pedro L.V. Peloso, Michelle L. Kortyna, J. Scott Keogh, Stephen C. Donnellan, Rachel Lockridge Mueller, Christopher J. Raxworthy, Krushnamegh Kunte, Santiago R. Ron, Sandeep Das, Nikhil Gaitonde, David M. GreenJim Labisko, Jing Che, David W. Weisrock

Research output: Contribution to journalArticlepeer-review

107 Scopus citations

Abstract

Molecular phylogenies have yielded strong support for many parts of the amphibian Tree of Life, but poor support for the resolution of deeper nodes, including relationships among families and orders. To clarify these relationships, we provide a phylogenomic perspective on amphibian relationships by developing a taxon-specific Anchored Hybrid Enrichment protocol targeting hundreds of conserved exons which are effective across the class. After obtaining data from 220 loci for 286 species (representing 94% of the families and 44% of the genera), we estimate a phylogeny for extant amphibians and identify gene tree-species tree conflict across the deepest branches of the amphibian phylogeny. We perform locus-by-locus genealogical interrogation of alternative topological hypotheses for amphibian monophyly, focusing on interordinal relationships. We find that phylogenetic signal deep in the amphibian phylogeny varies greatly across loci in a manner that is consistent with incomplete lineage sorting in the ancestral lineage of extant amphibians. Our results overwhelmingly support amphibian monophyly and a sister relationship between frogs and salamanders, consistent with the Batrachia hypothesis. Species tree analyses converge on a small set of topological hypotheses for the relationships among extant amphibian families. These results clarify several contentious portions of the amphibian Tree of Life, which in conjunction with a set of vetted fossil calibrations, support a surprisingly younger timescale for crown and ordinal amphibian diversification than previously reported. More broadly, our study provides insight into the sources, magnitudes, and heterogeneity of support across loci in phylogenomic data sets.

Original languageEnglish
Pages (from-to)49-66
Number of pages18
JournalSystematic Biology
Volume70
Issue number1
DOIs
StatePublished - Jan 1 2021

Bibliographical note

Publisher Copyright:
© The Author(s) 2020. Published by Oxford University Press on behalf of the Society of Systematic Biologists. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords

  • AIC
  • Amphibia
  • Batrachia
  • Gene tree-species tree discordance
  • Genomics
  • Information theory
  • Phylogeny

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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