We showed earlier that life spans of murine B lymphocytes could be estimated by measuring the functional reactivities of normal B cells upon transfer into x-linked immunodeficient (xid) mice, which do not respond to anti-mouse IgM (anti-μ) antibodies and thymic-independent type -2 (TI-2) antigens. Here the same approach was adopted, to evaluate the life spans of B-lymphocytes from aged mice. Spleen cells from normal young and aged mice were transferred into young or aged xid recipients and the decay kinetics were followed by measuring the proliferative response to anti-μ and PFC response to TNP-Ficoll, a prototype TI-2 antigen. The results indicated that anti-μ reactive B cells of both young and aged mice decayed with similar non-linear kinetics. About 50% of the donor cells decayed in 8-10 days, whereas, the remaining decayed at a slower rate and it appeared that the median life-expectancy of this latter population could be at least 3 weeks. Essentially, there was no apparent difference in the decay kinetics of anti-μ reactive B cells of young and aged mice. Unlike anti-μ reactive cells, TNP-Ficoll reactive B cells showed 2-3-fold enhancement in the PFC response during the first 2 weeks, and persisted at least until 5 weeks post transfer. This result indicated that TNP-Ficoll reactive B cells are long-lived. Further, it was found that the turn over rate of TNP-Ficoll reactive B.
|Number of pages||13|
|Journal||Mechanisms of Ageing and Development|
|State||Published - Dec 2 1991|
Bibliographical noteFunding Information:
This work was supported in part by the grants AI 21490, AG 05731 and a career research development award K04 AG 00422 (to B.S.) from the National Institute of Health, V.U. was supported in part by a postdoctoral fellowship from the PSP funds of the University of Kentucky. We thank Dr. H.E. McKean for statistical analysis of the data, Dr. E.C. Snow for critically reviewing this manuscript. Our thanks are also due to Ms. J. Morris for her expert technical assistance and Ms. Charlotte Bur-chett for her excellent secretarial assistance in the preparation of this manuscript.
ASJC Scopus subject areas
- Developmental Biology