PIB binding in aged primate brain: Enrichment of high-affinity sites in humans with Alzheimer's disease

Rebecca F. Rosen, Lary C. Walker, Harry LeVine

Research output: Contribution to journalArticlepeer-review

73 Scopus citations


Aged nonhuman primates accumulate large amounts of human-sequence amyloid β (Aβ) in the brain, yet they do not manifest the full phenotype of Alzheimer's disease (AD). To assess the biophysical properties of Aβ that might govern its pathogenic potential in humans and nonhuman primates, we incubated the benzothiazole imaging agent Pittsburgh Compound B (PIB) with cortical tissue homogenates from normal aged humans, humans with AD, and from aged squirrel monkeys, rhesus monkeys, and chimpanzees with cerebral Aβ-amyloidosis. Relative to humans with AD, high-affinity PIB binding is markedly reduced in cortical extracts from aged nonhuman primates containing levels of insoluble Aβ similar to those in AD. The high-affinity binding of PIB may be selective for a pathologic, human-specific conformation of multimeric Aβ, and thus could be a useful experimental tool for clarifying the unique predisposition of humans to Alzheimer's disease.

Original languageEnglish
Pages (from-to)223-234
Number of pages12
JournalNeurobiology of Aging
Issue number2
StatePublished - Feb 2011

Bibliographical note

Funding Information:
We thank Brian Ciliax, M. Paul Murphy and Jorge Ghiso for helpful discussions and technical expertise, Marla Gearing, Todd Preuss, Mary Lou Voytko, Amy Arnsten, Douglas Rosene and Daniel Anderson for generously providing tissue, and Jeromy Dooyema, Aaron Farberg and Carolyn Suwyn for excellent technical assistance. Funding was provided by the University Research Committee of Emory University, RR-00165, PO1AG026423, P50AG025688 and the Woodruff Foundation.


  • Alzheimer's disease
  • Benzothiazole
  • Cerebral amyloid angiopathy
  • Imaging
  • Nonhuman primates
  • Pittsburgh Compound B
  • Senile plaques
  • β-Amyloid

ASJC Scopus subject areas

  • Neuroscience (all)
  • Aging
  • Clinical Neurology
  • Developmental Biology
  • Geriatrics and Gerontology


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