Piezoelectric substrates promote neurite growth in rat spinal cord neurons

Núria Royo-Gascon; Michael Wininger; Jerry I. Scheinbeim; Bonnie L. Firestein; William Craelius

doi:10.1007/s10439-012-0628-y

Piezoelectric substrates promote neurite growth in rat spinal cord neurons

Núria Royo-Gascon
, Michael Wininger
, Jerry I. Scheinbeim
, Bonnie L. Firestein
, William Craelius

Pharmacology and Nutritional Sciences

Research output: Contribution to journal › Article › peer-review

109 Scopus citations

Abstract

We tested the possibility that exogenous electrical activity from a piezoelectric substrate could influence neuronal structure in cultured spinal cord neurons. Oscillating electrical fields were delivered to rat neurons via substrates consisting of poly(vinylidene fluoride) film, both in its piezoelectric (PZ) and non-piezoelectric (PV) forms. To induce oscillating electrical fields at the film surfaces, a 50 Hz mechanical vibration was applied. After 4 days of mechano-electrical stimulation, neuronal densities were increased by 115% and neurons grew 79% more neurites, with more than double the branch points, compared with neurons grown on non-stimulated PZ films (p < 0.001). The effects were due to electrical field, because vibration applied to non-PZ films did not increase neurite growth. We conclude that the oscillating electric fields produced from PZ polymer substrates can induce plastic changes in neurons of the central nervous system and herein we show its influence on neurite growth and branching.

Original language	English
Pages (from-to)	112-122
Number of pages	11
Journal	Annals of Biomedical Engineering
Volume	41
Issue number	1
DOIs	https://doi.org/10.1007/s10439-012-0628-y
State	Published - Jan 2013

Funding

We thank Dr. Nadarajah Vasanthan for chemical analyses, Dr. Jennifer Lynch for assistance, and Rocío Naveiras Cabello for image analysis. The NJ Commission on Spinal Cord Research provided the Fellowship 07-2932-SCR-E-0 to N.R., the NSF-IGERT Fellowship DGE 033196 was provided to M.W., and NSF grants IBN-0919747 and IBN-0548543, March of Dimes Foundation Grants 1-FY04-107, and NJCSCR grant # 07-3070-SCR-E-0 to B.L.F.

Funders	Funder number
NSF-IGERT	DGE 033196
March of Dimes Research Foundation	1-FY04-107
National Science Foundation	IBN-0919747, 0548543
NJ Commission on Spinal Cord Research	07-2932-SCR-E-0
NJCSCR	07-3070-SCR-E-0

Keywords

Electrical field
Growth factors
Neuron
Neuroplastic
Piezoelectric
Poly(vinylidene fluoride)
Polymer
PVDF

ASJC Scopus subject areas

Biomedical Engineering

Access to Document

10.1007/s10439-012-0628-y

Cite this

@article{5541dd4030274b37b9b1c737eb614ff0,

title = "Piezoelectric substrates promote neurite growth in rat spinal cord neurons",

abstract = "We tested the possibility that exogenous electrical activity from a piezoelectric substrate could influence neuronal structure in cultured spinal cord neurons. Oscillating electrical fields were delivered to rat neurons via substrates consisting of poly(vinylidene fluoride) film, both in its piezoelectric (PZ) and non-piezoelectric (PV) forms. To induce oscillating electrical fields at the film surfaces, a 50 Hz mechanical vibration was applied. After 4 days of mechano-electrical stimulation, neuronal densities were increased by 115\% and neurons grew 79\% more neurites, with more than double the branch points, compared with neurons grown on non-stimulated PZ films (p < 0.001). The effects were due to electrical field, because vibration applied to non-PZ films did not increase neurite growth. We conclude that the oscillating electric fields produced from PZ polymer substrates can induce plastic changes in neurons of the central nervous system and herein we show its influence on neurite growth and branching.",

keywords = "Electrical field, Growth factors, Neuron, Neuroplastic, Piezoelectric, Poly(vinylidene fluoride), Polymer, PVDF",

author = "N{\'u}ria Royo-Gascon and Michael Wininger and Scheinbeim, \{Jerry I.\} and Firestein, \{Bonnie L.\} and William Craelius",

year = "2013",

month = jan,

doi = "10.1007/s10439-012-0628-y",

language = "English",

volume = "41",

pages = "112--122",

number = "1",

}

TY - JOUR

T1 - Piezoelectric substrates promote neurite growth in rat spinal cord neurons

AU - Royo-Gascon, Núria

AU - Wininger, Michael

AU - Scheinbeim, Jerry I.

AU - Firestein, Bonnie L.

AU - Craelius, William

PY - 2013/1

Y1 - 2013/1

N2 - We tested the possibility that exogenous electrical activity from a piezoelectric substrate could influence neuronal structure in cultured spinal cord neurons. Oscillating electrical fields were delivered to rat neurons via substrates consisting of poly(vinylidene fluoride) film, both in its piezoelectric (PZ) and non-piezoelectric (PV) forms. To induce oscillating electrical fields at the film surfaces, a 50 Hz mechanical vibration was applied. After 4 days of mechano-electrical stimulation, neuronal densities were increased by 115% and neurons grew 79% more neurites, with more than double the branch points, compared with neurons grown on non-stimulated PZ films (p < 0.001). The effects were due to electrical field, because vibration applied to non-PZ films did not increase neurite growth. We conclude that the oscillating electric fields produced from PZ polymer substrates can induce plastic changes in neurons of the central nervous system and herein we show its influence on neurite growth and branching.

AB - We tested the possibility that exogenous electrical activity from a piezoelectric substrate could influence neuronal structure in cultured spinal cord neurons. Oscillating electrical fields were delivered to rat neurons via substrates consisting of poly(vinylidene fluoride) film, both in its piezoelectric (PZ) and non-piezoelectric (PV) forms. To induce oscillating electrical fields at the film surfaces, a 50 Hz mechanical vibration was applied. After 4 days of mechano-electrical stimulation, neuronal densities were increased by 115% and neurons grew 79% more neurites, with more than double the branch points, compared with neurons grown on non-stimulated PZ films (p < 0.001). The effects were due to electrical field, because vibration applied to non-PZ films did not increase neurite growth. We conclude that the oscillating electric fields produced from PZ polymer substrates can induce plastic changes in neurons of the central nervous system and herein we show its influence on neurite growth and branching.

KW - Electrical field

KW - Growth factors

KW - Neuron

KW - Neuroplastic

KW - Piezoelectric

KW - Poly(vinylidene fluoride)

KW - Polymer

KW - PVDF

UR - https://www.scopus.com/pages/publications/84871628980

UR - https://www.scopus.com/pages/publications/84871628980#tab=citedBy

U2 - 10.1007/s10439-012-0628-y

DO - 10.1007/s10439-012-0628-y

M3 - Article

C2 - 22864823

AN - SCOPUS:84871628980

SN - 0090-6964

VL - 41

SP - 112

EP - 122

JO - Annals of Biomedical Engineering

JF - Annals of Biomedical Engineering

IS - 1

ER -

Piezoelectric substrates promote neurite growth in rat spinal cord neurons

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this