Piglet immunization with a spike subunit vaccine enhances disease by porcine epidemic diarrhea virus

Jieshi Yu; Chithra Sreenivasan; Tirth Uprety; Rongyuan Gao; Chen Huang; Ella J. Lee; Steven Lawson; Julie Nelson; Jane Christopher-Hennings; Radhey S. Kaushik; Eric Nelson; Diego G. Diel; Ben M. Hause; Feng Li; Dan Wang

doi:10.1038/s41541-021-00283-x

Piglet immunization with a spike subunit vaccine enhances disease by porcine epidemic diarrhea virus

Jieshi Yu
, Chithra Sreenivasan
, Tirth Uprety
, Rongyuan Gao
, Chen Huang
, Ella J. Lee
, Steven Lawson
, Julie Nelson
, Jane Christopher-Hennings
, Radhey S. Kaushik
, Eric Nelson
, Diego G. Diel
, Ben M. Hause
, Feng Li
, Dan Wang

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Immunization with an insect cell lysate/baculovirus mixture containing recombinant porcine epidemic diarrhea virus (PEDV) spike protein induced high levels of neutralizing antibodies in both mice and piglets. However, immunization of piglets with this vaccine resulted in enhancement of disease symptoms and virus replication in vaccine recipients exposed to PEDV challenge. Thus, these observations demonstrate a previously unrecognized challenge of PEDV vaccine research, which has important implications for coronavirus vaccine development.

Original language	English
Article number	22
Journal	npj Vaccines
Volume	6
Issue number	1
DOIs	https://doi.org/10.1038/s41541-021-00283-x
State	Published - Dec 2021

Bibliographical note

Publisher Copyright:
© 2021, The Author(s).

Funding

We thank Katherine Meyer for her excellent assistance in animal experiments. The work was supported by USDA/NIFA 2016-67016-24949, Kentucky Agricultural Experiment Station, and William Robert Mills Endowment Fund.

Funders	Funder number
William Robert Mills Endowment Fund
U.S. Department of Agriculture
US Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative	2016-67016-24949
US Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative
Kentucky Agricultural Experiment Station

ASJC Scopus subject areas

Immunology
Pharmacology
Infectious Diseases
Pharmacology (medical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/s41541-021-00283-x

Cite this

Yu, J., Sreenivasan, C., Uprety, T., Gao, R., Huang, C., Lee, E. J., Lawson, S., Nelson, J., Christopher-Hennings, J., Kaushik, R. S., Nelson, E., Diel, D. G., Hause, B. M., Li, F., & Wang, D. (2021). Piglet immunization with a spike subunit vaccine enhances disease by porcine epidemic diarrhea virus. npj Vaccines, 6(1), Article 22. https://doi.org/10.1038/s41541-021-00283-x

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abstract = "Immunization with an insect cell lysate/baculovirus mixture containing recombinant porcine epidemic diarrhea virus (PEDV) spike protein induced high levels of neutralizing antibodies in both mice and piglets. However, immunization of piglets with this vaccine resulted in enhancement of disease symptoms and virus replication in vaccine recipients exposed to PEDV challenge. Thus, these observations demonstrate a previously unrecognized challenge of PEDV vaccine research, which has important implications for coronavirus vaccine development.",

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AU - Lee, Ella J.

AU - Lawson, Steven

AU - Nelson, Julie

AU - Christopher-Hennings, Jane

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Piglet immunization with a spike subunit vaccine enhances disease by porcine epidemic diarrhea virus

Abstract

Bibliographical note

Funding

ASJC Scopus subject areas

UN SDGs

Access to Document

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