PiRNA-Associated Germline Nuage Formation and Spermatogenesis Require MitoPLD Profusogenic Mitochondrial-Surface Lipid Signaling

Huiyan Huang, Qun Gao, Xiaoxue Peng, Seok Yong Choi, Krishna Sarma, Hongmei Ren, Andrew J. Morris, Michael A. Frohman

Research output: Contribution to journalArticlepeer-review

225 Scopus citations

Abstract

The mammalian Phospholipase D MitoPLD facilitates mitochondrial fusion by generating the signaling lipid phosphatidic acid (PA). The Drosophila MitoPLD homolog Zucchini (Zuc), a proposed cytoplasmic nuclease, is required for piRNA generation, a critical event in germline development. We show that Zuc localizes to mitochondria and has MitoPLD-like activity. Conversely, MitoPLD-/- mice exhibit the meiotic arrest, DNA damage, and male sterility characteristic of mice lacking piRNAs. The primary function of MitoPLD seems to be the generation of mitochondrial-surface PA. This PA in turn recruits the phosphatase Lipin 1, which converts PA to diacylglycerol and promotes mitochondrial fission, suggesting a mechanism for mitochondrial morphology homeostasis. MitoPLD and Lipin 1 have opposing effects on mitochondria length and on intermitochondrial cement (nuage), a structure found between aggregated mitochondria that is implicated in piRNA generation. We propose that mitochondrial-surface PA generated by MitoPLD/Zuc recruits or activates nuage components critical for piRNA production.

Original languageEnglish
Pages (from-to)376-387
Number of pages12
JournalDevelopmental Cell
Volume20
Issue number3
DOIs
StatePublished - Mar 15 2011

Bibliographical note

Funding Information:
We thank Drs. S. Chuma (Kyoto U.), R. Jessberger (Dresden U. Tech.), A. Vasileva (Mt. Sinai), S. K-Miyagawa and T. Nakano (Osaka U.), and C. Chen and T. Pawson (Samuel Lunenfeld Res. Inst.) for the kind gifts of piRNA pathway expression plasmids, Dr. A. Newton for the DAG sensor, B. Cyge for advice, Y. Altshuller for generating innumerable plasmid constructs, and many colleagues for critical manuscript feedback. Supported by NIH (GM071520, GM084251 to M.A.F.; GM50388, P20RR021954 to A.J.M.) and fellowships from NIH (T32-DK07521) and AHA to Q.G. and H.R.

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology (all)
  • Developmental Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'PiRNA-Associated Germline Nuage Formation and Spermatogenesis Require MitoPLD Profusogenic Mitochondrial-Surface Lipid Signaling'. Together they form a unique fingerprint.

Cite this